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Literature summary extracted from

  • Kramer, G.; Ramachandiran, V.; Horowitz, P.; Hardesty, B.
    An additional serine residue at the C terminus of rhodanese destabilizes the enzyme (2001), Arch. Biochem. Biophys., 385, 332-337.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.1 expression in a cell free Escherichia coli system by coupled transcription/translation Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.1 Arg one additional Arg residue at the 3'-end followed by a TAA stop codon, no significant changes to the wild-type enzyme Bos taurus
2.8.1.1 Ser-1 one additional Ser residue at the 3'-end followed by a TAA stop codon, loss of enzymatic activity above 30°C Bos taurus
2.8.1.1 Ser-2 one additional Ser residue at the 3'-end followed by a TAG stop codon, loss of enzymatic activity above 30°C Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Bos taurus
-
sulfite + thiocyanate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Bos taurus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.1 332
-
wild-type Bos taurus
2.8.1.1 353
-
Ser-1 Bos taurus
2.8.1.1 389
-
Ser-2 Bos taurus
2.8.1.1 391
-
one additional Arg residue at the 3'-end followed by a TAA stop codon Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 thiosulfate + cyanide
-
Bos taurus sulfite + thiocyanate
-
?