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Literature summary extracted from

  • Miller-Martin, D.M.; Chirgwin, J.M.; Horowitz, P.M.
    Mutations of noncatalytic sulfhydryl groups influence the stability, folding, and oxidative susceptibility of rhodanese (1994), J. Biol. Chem., 269, 3423-3428.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.1 expression in Escherichia coli Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.1 C247S no activity to thiosulfate Bos taurus
2.8.1.1 C254S more resistant than wild-type to inactivation by dithiothreitol, more readily reactivated following oxidative inactivation and increased exposure of hydrophobic surfaces following removal of the transferable sulfur Bos taurus
2.8.1.1 C254S/C263S less stable in urea than wild type, more resistant than wild-type to inactivation by dithiothreitol, more readily reactivated following oxidative inactivation and increased exposure of hydrophobic surfaces following removal of the transferable sulfur Bos taurus
2.8.1.1 C263S less stable in urea than wild type Bos taurus
2.8.1.1 C63S less stable in urea than wild type Bos taurus
2.8.1.1 C63S/C254S/C263S less stable in urea than wild type, more resistant than wild-type to inactivation by dithiothreitol, more readily reactivated following oxidative inactivation and increased exposure of hydrophobic surfaces following removal of the transferable sulfur Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Bos taurus
-
 sulfite + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Bos taurus
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.8.1.1
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 thiosulfate + cyanide
-
 Bos taurus sulfite + thiocyanate
-
 ?