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Literature summary extracted from

  • Luo, G.X.; Horowitz, P.M.
    The sulfurtransferase activity and structure of rhodanese are affected by site-directed replacement of Arg-186 or Lys-249 (1994), J. Biol. Chem., 269, 8220-8225.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.8.1.1 expression in Escherichia coli Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
2.8.1.1 K249A reduced stability, comparable secondary structure, more easily exposed hydrophobic surfaces and a core structure that denatured similarly to the wild-type enzyme, inactive using thiosulfate, but active with thiosulfonates Bos taurus
2.8.1.1 R186L increased KM for thiosulfate Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.1 3.7
-
thiosulfate pH 7.5, wild-type Bos taurus
2.8.1.1 73.2
-
thiosulfate pH 7.5, R186L Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Bos taurus
-
sulfite + thiocyanate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Bos taurus
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.8.1.1 refolds from 8 M urea to enzymatically active species Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.8.1.1 575.5
-
R186L Bos taurus
2.8.1.1 672.8
-
wild-type Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 cyanide + 2-aminoethanethiosulfate K249A Bos taurus ?
-
?
2.8.1.1 cyanide + ethanethiosulfate K249A Bos taurus ?
-
?
2.8.1.1 cyanide + p-toluenethiosulfonate K249A Bos taurus ?
-
?
2.8.1.1 thiosulfate + cyanide
-
Bos taurus sulfite + thiocyanate
-
?