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Literature summary extracted from
- Recombinant bovine rhodanese: purification and comparison with bovine liver rhodanese (1992), Biochim. Biophys. Acta, 1121, 286-292.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.8.1.1 | expression in Escherichia coli BL21(DE3) | Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.8.1.1 | 15.9 | - |
thiosulfate | liver rhodanese | Bos taurus |  |
| 2.8.1.1 | 16.8 | - |
thiosulfate | recombinant rhodanese | Bos taurus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.1 | 33000 | - |
x * 33000, bovine liver and recombinant Escherichia coli BL21(DE3) enzyme, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.1.1 | thiosulfate + cyanide | Bos taurus | - |
sulfite + thiocyanate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.8.1.1 | Bos taurus | - |
bovine liver and recombinant Escherichia coli BL21(DE3) enzyme | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.8.1.1 | bovine liver and recombinant Escherichia coli BL21(DE3) enzyme | Bos taurus |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 2.8.1.1 | refolds from 8 M urea to enzymatically active species | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.8.1.1 | liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.8.1.1 | 730 | - |
purificated liver rhodanese | Bos taurus |
| 2.8.1.1 | 816 | - |
washed precipitate of recombinant rhodanese | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.8.1.1 | thiosulfate + cyanide | - |
Bos taurus | sulfite + thiocyanate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.8.1.1 | ? | x * 33000, bovine liver and recombinant Escherichia coli BL21(DE3) enzyme, SDS-PAGE | Bos taurus |
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