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Literature summary extracted from

  • Wrobel, M.; Frendo, J.
    Comparison of some molecular and catalytic properties of mitochondrial and cytosolic rhodanese and mercaptopyruvate sulphurtransferase from frog (Rana temporaria) liver (1984), Bull. Pol. Acad. Sci. Biol. Sci., 32, 303-313.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.1 26.9
-
 thiosulfate mitochondrial enzyme Rana temporaria
2.8.1.2 7.3
-
 3-Mercaptopyruvate cytosolic enzyme Rana temporaria
2.8.1.2 7.6
-
 3-Mercaptopyruvate mitochondrial enzyme Rana temporaria

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.1 cytosol
-
 Rana temporaria 5829
-
2.8.1.1 mitochondrion
-
 Rana temporaria 5739
-
2.8.1.2 cytosol
-
 Rana temporaria 5829
-
2.8.1.2 mitochondrion
-
 Rana temporaria 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.1 9000
-
 gel filtration, mitochondria Rana temporaria
2.8.1.1 20300
-
 gel filtration, cytosol Rana temporaria
2.8.1.2 10900
-
 mitochondria, gel filtration Rana temporaria
2.8.1.2 30200
-
 cytosol, gel filtration Rana temporaria

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.8.1.1 thiosulfate + cyanide Rana temporaria
-
 sulfite + thiocyanate
-
 ?
2.8.1.2 3-mercaptopyruvate + cyanide Rana temporaria
-
 pyruvate + thiocyanate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Rana temporaria
-
-
-
2.8.1.2 Rana temporaria
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.1.1 partial Rana temporaria
2.8.1.2 partial Rana temporaria

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.8.1.1 liver
-
 Rana temporaria
-
2.8.1.2 liver
-
 Rana temporaria
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 thiosulfate + cyanide
-
 Rana temporaria sulfite + thiocyanate
-
 ?
2.8.1.2 3-mercaptopyruvate + cyanide
-
 Rana temporaria pyruvate + thiocyanate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.8.1.1 30
-
 mitochondria Rana temporaria
2.8.1.1 35
-
 cytosol Rana temporaria

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.8.1.1 6.5
-
 mitochondria Rana temporaria
2.8.1.1 7.5
-
 cytosol Rana temporaria

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.8.1.1 Rana temporaria isoelectrophoresis in polyacrylamide gels, cytosol
-
 5
2.8.1.1 Rana temporaria isoelectrophoresis in polyacrylamide gels, mitochondria
-
 5.3