Literature summary extracted from
Kim, I.Y.; Guimaraes, M.J.; Zlotnik, A.; Bazan, J.F.; Stadtman, T.C.
Fetal mouse selenophosphate synthetase 2 (SPS2): Characterization of the cysteine mutant form overproduced in a baculovirus-insect cell system (1997), Proc. Natl. Acad. Sci. USA, 94, 418-421.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.9.3 |
expression in Spodoptera frugiperda Sf9 insect cells via baculovirus infection, expression as N-FLAG tagged protein |
Mus musculus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.9.3 |
Na+ |
5-20 mM Na+ has little effect on enzyme activity in the presence or absence of 1.5 mM K+ |
Mus musculus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.9.3 |
Mg2+ |
required |
Mus musculus |
|
2.7.9.3 |
Mg2+ |
the optimal range for the molar ratio of Mg2+ to ATP is between 2:1 and 4:1 |
Mus musculus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.9.3 |
Mus musculus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.9.3 |
recombinant from Sf9 insect cells as N-FLAG tagged protein |
Mus musculus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.9.3 |
ATP + selenide + H2O |
specific for ATP |
Mus musculus |
AMP + selenophosphate + phosphate |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.7.9.3 |
37 |
- |
assay at |
Mus musculus |