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Literature summary extracted from

  • Ye, D.; Wei, M.; McGuire, M.; Huang, K.; Kapadia, G.; Herzberg, O.; Martin, B.M.; Dunaway-Mariano, D.
    Investigation of the catalytic site within the ATP-grasp domain of Clostridium symbiosum pyruvate phosphate dikinase (2001), J. Biol. Chem., 276, 37630-37639.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.9.1 D280A partial activity [Clostridium] symbiosum
2.7.9.1 E279A partial activity [Clostridium] symbiosum
2.7.9.1 R135A partial activity, 15 mutant enzymes studied [Clostridium] symbiosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.1 5'-adenylimidodiphosphate
-
 [Clostridium] symbiosum
2.7.9.1 adenosine
-
 [Clostridium] symbiosum
2.7.9.1 adenosine 5'-mono-O-phosphorothiolate
-
 [Clostridium] symbiosum
2.7.9.1 AMP
-
 [Clostridium] symbiosum
2.7.9.1 GMP noncompetitive to ATP [Clostridium] symbiosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.1 0.009
-
 AMP pH 6.8, 25ºC, pyruvate-producing reaction, values for 7 mutant PPDK [Clostridium] symbiosum
2.7.9.1 0.027
-
 phosphoenolpyruvate pH 6.8, 25ºC, pyruvate-producing reaction, values for 7 mutant PPDK [Clostridium] symbiosum
2.7.9.1 0.081
-
 ATP pH 7, 25ºC, E279A mutant, phosphoenolpyruvate-producing reaction [Clostridium] symbiosum
2.7.9.1 0.089
-
 diphosphate pH 6.8, 25ºC, pyruvate-producing reaction, values for 7 mutant PPDK [Clostridium] symbiosum
2.7.9.1 0.1
-
 2'-dAMP pH 6.8, 25ºC [Clostridium] symbiosum
2.7.9.1 0.19
-
 ATP pH 7, 25ºC, wild type and D280 mutant, phosphoenolpyruvate-producing reaction [Clostridium] symbiosum
2.7.9.1 0.25
-
 3'-dATP pH 7, 25ºC [Clostridium] symbiosum
2.7.9.1 0.28
-
 2',3'-dideoxyadenosine 5'-triphosphate pH 7, 25ºC [Clostridium] symbiosum
2.7.9.1 0.35
-
 2'-dATP pH 7, 25ºC [Clostridium] symbiosum
2.7.9.1 0.41
-
 ATP pH 7, 25ºC, R135A mutant, phosphoenolpyruvate-producing reaction [Clostridium] symbiosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 [Clostridium] symbiosum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 2',3'-dideoxyadenosine 5'-triphosphate + pyruvate + phosphate
-
 [Clostridium] symbiosum ?
-
 r
2.7.9.1 2'-dAMP + phosphoenolpyruvate + diphosphate
-
 [Clostridium] symbiosum 2'-dATP + pyruvate + phosphate
-
 ?
2.7.9.1 2'-dATP + pyruvate + phosphate
-
 [Clostridium] symbiosum ?
-
 r
2.7.9.1 3'-dATP + pyruvate + phosphate
-
 [Clostridium] symbiosum ?
-
 r

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.9.1 0.07
-
 5'-adenylimidodiphosphate pH 6.8, 25ºC [Clostridium] symbiosum
2.7.9.1 0.31
-
 adenosine 5'-mono-O-phosphorothiolate pH 6.8, 25ºC [Clostridium] symbiosum
2.7.9.1 0.32
-
 adenosine pH 6.8, 25ºC [Clostridium] symbiosum
2.7.9.1 8.7
-
 GMP pH 6.8, 25ºC [Clostridium] symbiosum