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Literature summary extracted from

  • Goss, N.H.; Wood, H.G.
    Covalent chemistry of pyruvate, orthophosphate dikinase (1982), Methods Enzymol., 87, 51-66.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.1 Bromopyruvate irreversible inactivation [Clostridium] symbiosum
2.7.9.1 Bromopyruvate
-
 Propionibacterium freudenreichii subsp. shermanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 94000
-
 2 * 94000, SDS-PAGE [Clostridium] symbiosum
2.7.9.1 170000
-
 gel filtration [Clostridium] symbiosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Entamoeba histolytica
-
-
-
2.7.9.1 Komagataeibacter xylinus
-
-
-
2.7.9.1 Propionibacterium freudenreichii subsp. shermanii
-
-
-
2.7.9.1 [Clostridium] symbiosum
-
 ATCC 14940
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.1 partial, using ammonium sulfate precipitation, and chromatography on DEAE-cellulose and Bio-Gel [Clostridium] symbiosum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.9.1 ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate mechanism Entamoeba histolytica
a
2.7.9.1 ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate mechanism Propionibacterium freudenreichii subsp. shermanii
a
2.7.9.1 ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate mechanism Komagataeibacter xylinus
a
2.7.9.1 ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate mechanism [Clostridium] symbiosum
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.1 additional information
-
-
 Entamoeba histolytica
2.7.9.1 additional information
-
-
 Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 additional information
-
-
 Komagataeibacter xylinus
2.7.9.1 additional information
-
-
 [Clostridium] symbiosum
2.7.9.1 29.4
-
-
 [Clostridium] symbiosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate
-
 Entamoeba histolytica AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 ATP + pyruvate + phosphate
-
 Propionibacterium freudenreichii subsp. shermanii AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 ATP + pyruvate + phosphate
-
 Komagataeibacter xylinus AMP + phosphoenolpyruvate + diphosphate
-
 ?
2.7.9.1 ATP + pyruvate + phosphate
-
 [Clostridium] symbiosum AMP + phosphoenolpyruvate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.9.1 dimer 2 * 94000, SDS-PAGE [Clostridium] symbiosum