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Literature summary extracted from

  • Milner, Y.; Michaels, G.; Wood, H.G.
    Pyruvate,phosphate dikinase from Bacteroides symbiosus and Propionibacterium shermanii (1975), Methods Enzymol., 42C, 199-212.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.9.1 freezing and thawing inactivates [Clostridium] symbiosum
2.7.9.1 sensitive to dilution, particularly at concentrations below 0.3 mg/ml, stable to freezing and thawing Propionibacterium freudenreichii subsp. shermanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.1 5,5-dithiobis(2-nitrobenzoic acid)
-
[Clostridium] symbiosum
2.7.9.1 5,5-dithiobis(2-nitrobenzoic acid)
-
Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 alpha-beta-methylene ATP competitive to ATP [Clostridium] symbiosum
2.7.9.1 alpha-beta-methylene ATP competitive to ATP Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 beta-gamma-methylene ATP competitive to ATP [Clostridium] symbiosum
2.7.9.1 beta-gamma-methylene ATP competitive to ATP Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 Bromopyruvate competitive to phosphoenolpyruvate [Clostridium] symbiosum
2.7.9.1 Bromopyruvate competitive to phosphoenolpyruvate Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 Ca2+
-
[Clostridium] symbiosum
2.7.9.1 Ca2+ competitive to Mg2+ Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 CTP
-
[Clostridium] symbiosum
2.7.9.1 CTP
-
Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 dATP
-
[Clostridium] symbiosum
2.7.9.1 dATP
-
Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 Fe2+ competitive to Mg2+ Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 GMP competitive to ATP Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 GTP competitive to ATP Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 iodoacetate
-
[Clostridium] symbiosum
2.7.9.1 iodoacetate
-
Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 ITP
-
[Clostridium] symbiosum
2.7.9.1 ITP
-
Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 Methylene diphosphonate competitive to diphosphate [Clostridium] symbiosum
2.7.9.1 Methylene diphosphonate competitive to diphosphate Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 additional information not inhibited by iodoacetamide [Clostridium] symbiosum
2.7.9.1 additional information not inhibited by iodoacetamide Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 N-ethylmaleimide
-
[Clostridium] symbiosum
2.7.9.1 N-ethylmaleimide
-
Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 p-hydroxymercuribenzoate
-
[Clostridium] symbiosum
2.7.9.1 p-hydroxymercuribenzoate
-
Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 Phosphoglycolate competitive to phosphoenolpyruvate [Clostridium] symbiosum
2.7.9.1 Sulfhydryl agents
-
[Clostridium] symbiosum
2.7.9.1 Sulfhydryl agents
-
Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.1 0.0015
-
AMP pH 6.8, 25ºC Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 0.004
-
ATP pH 6.8, 25ºC Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 0.015
-
AMP pH 6.7, 25ºC [Clostridium] symbiosum
2.7.9.1 0.05
-
ATP pH 6.7, 25ºC [Clostridium] symbiosum
2.7.9.1 0.08
-
diphosphate pH 6.7, 25ºC [Clostridium] symbiosum
2.7.9.1 0.1
-
pyruvate pH 6.8, 25ºC Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 0.1
-
pyruvate pH 6.7, 25ºC [Clostridium] symbiosum
2.7.9.1 0.1
-
phosphoenolpyruvate pH 6.7, 25ºC [Clostridium] symbiosum
2.7.9.1 0.8
-
phosphate pH 6.7, 25ºC [Clostridium] symbiosum
2.7.9.1 1
-
phosphate pH 6.8, 25ºC Propionibacterium freudenreichii subsp. shermanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.1 Co2+
-
[Clostridium] symbiosum
2.7.9.1 K+
-
[Clostridium] symbiosum
2.7.9.1 Mg2+
-
[Clostridium] symbiosum
2.7.9.1 Mn2+
-
[Clostridium] symbiosum
2.7.9.1 NH4+
-
[Clostridium] symbiosum
2.7.9.1 Rb+ 25% of the activity relative to NH4+ [Clostridium] symbiosum
2.7.9.1 Tl+ 94% of the activity relative to NH4+ [Clostridium] symbiosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.9.1 75000
-
2 * 75000, SDS-PAGE [Clostridium] symbiosum
2.7.9.1 150000
-
gel filtration Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 150000 160000 equilibrium sedimentation and gel filtration [Clostridium] symbiosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Propionibacterium freudenreichii subsp. shermanii
-
-
-
2.7.9.1 [Clostridium] symbiosum
-
ATCC 14940
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.1 partial, using ammonium sulfate precipitation and chromatography on DEAE-cellulose and Sephadex G-200 Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 partial, using ammonium sulfate precipitation, and chromatography on DEAE-cellulose and Bio-Gel [Clostridium] symbiosum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.9.1 ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate mechanism Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate mechanism [Clostridium] symbiosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.1 2.1
-
-
Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 35
-
-
[Clostridium] symbiosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate specificity is strictly restricted to adenine nucleotides Propionibacterium freudenreichii subsp. shermanii AMP + phosphoenolpyruvate + diphosphate
-
?
2.7.9.1 ATP + pyruvate + phosphate specificity is strictly restricted to adenine nucleotides [Clostridium] symbiosum AMP + phosphoenolpyruvate + diphosphate
-
?
2.7.9.1 ATP + pyruvate + phosphate dAMP can replace ATP but with 20% of the activity [Clostridium] symbiosum AMP + phosphoenolpyruvate + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.7.9.1 dimer 2 * 75000, SDS-PAGE [Clostridium] symbiosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.9.1 25
-
assay at Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 25
-
assay at [Clostridium] symbiosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.9.1 6.4 6.7 pyruvate formation [Clostridium] symbiosum
2.7.9.1 6.5 7.5 phosphoenolpyruvate formation Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 6.8
-
pyruvate formation Propionibacterium freudenreichii subsp. shermanii
2.7.9.1 7 7.8 phosphoenolpyruvate formation [Clostridium] symbiosum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.9.1 6.5 6.7
-
Propionibacterium freudenreichii subsp. shermanii