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Literature summary extracted from

  • Reeves, R.E.
    Pyruvate,phosphate dikinase from Bacteroides symbiosus (1971), Biochem. J., 125, 531-539.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.9.1 does not require thiol compounds to maintain stability during storage or assay [Clostridium] symbiosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.1 CTP total inhibition of the forward reaction at 1 mM [Clostridium] symbiosum
2.7.9.1 dATP 40% inhibition at 1 mM [Clostridium] symbiosum
2.7.9.1 ITP total inhibition of the forward reaction at 1 mM [Clostridium] symbiosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.1 0.0035
-
 AMP pH 6.8, 25ºC, phosphoenolpyruvate formation [Clostridium] symbiosum
2.7.9.1 0.08
-
 pyruvate pH 6.8, 25ºC, pyruvate formation [Clostridium] symbiosum
2.7.9.1 0.1
-
 ATP pH 6.8, 25ºC, pyruvate formation [Clostridium] symbiosum
2.7.9.1 0.6
-
 phosphate pH 6.8, 25ºC, pyruvate formation [Clostridium] symbiosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.9.1 Co2+ 1 mM of this ion promotes pyruvate formation [Clostridium] symbiosum
2.7.9.1 K+ half-maximal activity with 20 mM potassium chloride at pH 6.8 [Clostridium] symbiosum
2.7.9.1 Mg2+ 1 mM of this ion promotes reaction in both directions [Clostridium] symbiosum
2.7.9.1 Mn2+ 1 mM of this ion promotes pyruvate formation [Clostridium] symbiosum
2.7.9.1 NH4+
-
 [Clostridium] symbiosum
2.7.9.1 Ni2+ 1 mM of this ion promotes pyruvate formation [Clostridium] symbiosum

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 [Clostridium] symbiosum
-
 ATCC 14940
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.9.1 partial, using ammonium sulfate precipitation, and chromatography on DEAE-cellulose and Bio-Gel [Clostridium] symbiosum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.9.1 additional information
-
-
 [Clostridium] symbiosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 ATP + pyruvate + phosphate specificity is strictly restricted to adenine nucleotides [Clostridium] symbiosum AMP + phosphoenolpyruvate + diphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.9.1 25
-
 assay at [Clostridium] symbiosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.9.1 6.4
-
 reverse reaction, pyruvate formation [Clostridium] symbiosum
2.7.9.1 7.2 7.8 forward reaction, phosphoenolpyruvate formation [Clostridium] symbiosum