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Literature summary extracted from

  • Lambalot, R.H.; Walsh, C.T.
    Holo-[acyl-carrier-protein] synthase of Escherichia coli (1997), Methods Enzymol., 279, 254-262.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.7 recombinant ACP synthase overproduced in Escherichia coli BL21(DE3)pACPS1 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.001
-
 apo-acyl-carrier protein pH 8.8, 37°C Escherichia coli
2.7.8.7 0.05
-
 CoA pH 8.8, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.7 14053
-
 2 * 14053, calculated from nucleotide sequence Escherichia coli
2.7.8.7 18470
-
 2 * 18470, calculated from molar extinction coefficient by the method of Gill and von Hippel Escherichia coli
2.7.8.7 28000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Escherichia coli
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7 recombinant enzyme, expressed in Escherichia coli BL21(DE3)pACPS1 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.7 0.32
-
 recombinant Escherichia coli BL21(DE3)pACPS1 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Escherichia coli adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] Dcp from Lactobacillus casei, NodF from Rhizobium leguminosarum and several polyketide synthase ACPs from Streptomyces sp. also serves as substrates Escherichia coli adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.8.7 homodimer 2 * 18470, calculated from molar extinction coefficient by the method of Gill and von Hippel Escherichia coli
2.7.8.7 homodimer 2 * 14053, calculated from nucleotide sequence Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.8.7 1.17
-
 apo-acyl-carrier protein pH 8.8, 37°C Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.8.7 Escherichia coli calculated from molar extinction coefficient by the method of Gill and von Hippel
-
 9.25