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Literature summary extracted from

  • Prescott, D.J.; Elovson, J.; Vagelos, P.R.
    Acyl carrier protein synthetase (1975), Methods Enzymol., 35B, 95-101.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.8.7 stable when CoA is present at half-saturating concentrations Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.7 apo-acyl-carrier protein substrate inhibition Escherichia coli
2.7.8.7 additional information no substrate inhibition by apo-peptide(1--74) Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.7 0.00055
-
 apo-acyl-carrier protein pH 8.0, 33°C Escherichia coli
2.7.8.7 0.002
-
 apo-peptide(1-->74)
-
 Escherichia coli
2.7.8.7 0.15
-
 CoA pH 8.0, 33°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.7 Mg2+
-
 Escherichia coli
2.7.8.7 Mn2+
-
 Escherichia coli
2.7.8.7 additional information Ca2+, Fe2+, Co2+ and Zn2+ are ineffective, inhibitory or both, at intermediate and high concentrations Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.7 50000
-
 gel filtration, sucrose density gradient sedimentation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein] Escherichia coli
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein] Escherichia coli B / ATCC 11303
-
 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.7 Escherichia coli
-
-
-
2.7.8.7 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.7
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.7 0.0079
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.8.7 -196°C, stored in liquid nitrogen, enzyme maintains full activity for at least 1 year Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Escherichia coli adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA + apo-[acyl-carrier protein]
-
 Escherichia coli B / ATCC 11303 adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
 r
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-peptide(1->74)
-
 Escherichia coli ?
-
 ?
2.7.8.7 CoA-[4'-phosphopantetheine] + apo-peptide(1->74)
-
 Escherichia coli B / ATCC 11303 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.7 7.5 9
-
 Escherichia coli