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Literature summary extracted from

  • Goldberg, D.E.; Rumley, M.K.; Kennedy, E.P.
    Biosynthesis of membrane-derived oligosaccharides: a periplasmic phosphoglyceroltransferase (1981), Proc. Natl. Acad. Sci. USA, 78, 5513-5517.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.21 cellobiose 5 mM, competitive to membrane-derived oligosaccharides, 77% of the activity without addition Escherichia coli
2.7.8.21 D-glucose 5 mM, competitive to membrane-derived oligosaccharides, 78% of the activity without addition Escherichia coli
2.7.8.21 D-maltose 5 mM, competitive to membrane-derived oligosaccharides, 82% of the activity without addition Escherichia coli
2.7.8.21 EDTA
-
 Escherichia coli
2.7.8.21 gentiobiose 5 mM, competitive to membrane-derived oligosaccharides, 15% of the activity without addition Escherichia coli
2.7.8.21 sophorose 5 mM, competitive to membrane-derived oligosaccharides, 56% of the activity without addition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.21 1
-
 gentiobiose pH 7.8, 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.21 periplasmic space
-
 Escherichia coli 42597
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.21 Ca2+ less effective than Mn2+ Escherichia coli
2.7.8.21 Co2+ less effective than Mn2+ Escherichia coli
2.7.8.21 Mg2+ less effective than Mn2+ Escherichia coli
2.7.8.21 Mn2+ absolute requirement for a divalent cation, Mn2+ most active Escherichia coli
2.7.8.21 additional information absolute requirement for a divalent cation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.21 56000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.21 membrane-derived oligosaccharides Escherichia coli
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.21 Escherichia coli
-
 K-12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.21 partial Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.21 D-gentiobiose
-
 Escherichia coli ?
-
 ?
2.7.8.21 membrane-derived oligosaccharides transfer of a glycerophospho group from one membrane-derived oligosaccharide to another. beta-Linked glucose residues in simple glucosides, such as gentiobiose, can act as acceptors Escherichia coli additional information in presence of low concentrations of acceptor, free cyclic 1,2-phosphoglycerol is formed ?
2.7.8.21 membrane-derived oligosaccharides
-
 Escherichia coli ?
-
 ?
2.7.8.21 additional information no activity detected with phosphatidylglycerol as donor, or suspensions of phospholipid mixed with Triton X-100 or in mixed vesicles with phosphatidylcholine Escherichia coli ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.21 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.21 7.8
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.8.21 6.5 7.8 active Escherichia coli