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Literature summary extracted from

  • Bao, M.; Elmendorf, B.J.; Booth, J.L.; Drake, R.R.; Canfield, W.M.
    Bovine UDP-N-acetylglucosamine:lysosomal-enzyme N-acetylglucosamine-1-phosphotransferase. II. Enzymatic characterization and identification of the catalytic subunit (1996), J. Biol. Chem., 271, 31446-31451.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.17 D-glucose 6-phosphate activates Bos taurus
2.7.8.17 D-mannose 6-phosphate activates Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.17 ADP 5 mM, 39% inhibition Bos taurus
2.7.8.17 ATP 5 mM, 59% inhibition Bos taurus
2.7.8.17 EDTA 10 mM, complete inhibition Bos taurus
2.7.8.17 additional information selective inhibition of cathepsin D phosphorylation by rabbit anti-bovine GlcNAc phosphotransferase Bos taurus
2.7.8.17 Sodium phosphate 5 mM, pH 7.0, 55% inhibition Bos taurus
2.7.8.17 UDP 5 mM, 74% inhibition Bos taurus
2.7.8.17 UDPglucose 5 mM, 66% inhibition; competitive Bos taurus
2.7.8.17 UTP 5 mM, 34% inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.17 0.018
-
 cathepsin D pH 7.4 Bos taurus
2.7.8.17 0.022
-
 uteroferrin pH 7.6 Bos taurus
2.7.8.17 0.03
-
 UDP-GlcNAc pH 7.6 Bos taurus
2.7.8.17 0.064
-
 methyl-alpha-D-mannoside pH 7.6 Bos taurus
2.7.8.17 1.244
-
 ribonuclease B pH 7.6 Bos taurus
2.7.8.17 63.7
-
 methyl-alpha-D-mannoside pH 7.6 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.17 Mg2+ strictly dependent on Mg2+ or Mn2+ Bos taurus
2.7.8.17 Mn2+ Km: 0.185 mM, strictly dependent on Mg2+ or Mn2+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.17 51000
-
 2 * 166000 + 2 * 51000 + 2 * 56000, a 540000 Da enzyme complex is composed of disulfide-linked homodimers of 166000 and 51000 Da subunits and two identical, noncovalently associated 56000 Da subunits, SDS-PAGE Bos taurus
2.7.8.17 56000
-
 2 * 166000 + 2 * 51000 + 2 * 56000, a 540000 Da enzyme complex is composed of disulfide-linked homodimers of 166000 and 51000 Da subunits and two identical, noncovalently associated 56000 Da subunits, SDS-PAGE Bos taurus
2.7.8.17 166000
-
 2 * 166000 + 2 * 51000 + 2 * 56000, a 540000 Da enzyme complex is composed of disulfide-linked homodimers of 166000 and 51000 Da subunits and two identical, noncovalently associated 56000 Da subunits, SDS-PAGE Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.17 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.17
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.17 mammary gland
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.17 UDP-N-acetyl-D-glucosamine + cathepsin D
-
 Bos taurus UMP + ?
-
 ?
2.7.8.17 UDP-N-acetyl-D-glucosamine + methyl-alpha-D-mannoside
-
 Bos taurus UMP + N-acetyl-D-glucosamine-phospho-(methyl-alpha-D-mannoside)
-
 ?
2.7.8.17 UDP-N-acetyl-D-glucosamine + ribonuclease B
-
 Bos taurus UMP + ?
-
 ?
2.7.8.17 UDP-N-acetyl-D-glucosamine + UDP-glucose
-
 Bos taurus UMP + ?
-
 ?
2.7.8.17 UDP-N-acetyl-D-glucosamine + uteroferrin
-
 Bos taurus UMP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.17 hexamer 2 * 166000 + 2 * 51000 + 2 * 56000, a 540000 Da enzyme complex is composed of disulfide-linked homodimers of 166000 and 51000 Da subunits and two identical, noncovalently associated 56000 Da subunits, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.17 6.6 7.5
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.8.17 0.733
-
 UDPglucose pH 7.6 Bos taurus