BRENDA - Enzyme Database

Developmental regulation and tissue-specific expression of hamster dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT)

Mota, O.M.; Huang, G.T.; Kukuruzinska, M.A.; Biochem. Biophys. Res. Commun. 204, 284-291 (1994)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
2.7.8.15
A263D
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
D252A
mutation does not allow plasmid shuffling, indicating that the residues is essential for activity
Cricetulus griseus
2.7.8.15
D252A/F254I
mutation does not allow plasmid shuffling, indicating that the residues are essential for activity
Cricetulus griseus
2.7.8.15
D252A/L102F
mutation does not allow plasmid shuffling, indicating that the residues are essential for activity
Cricetulus griseus
2.7.8.15
F249A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
F257A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
K125A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
N182A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
N182A/I186M
mutation does not allow plasmid shuffling, indicating that the residues are essential for activity
Cricetulus griseus
2.7.8.15
N182A/W122R
mutation does not allow plasmid shuffling, indicating that the residues are essential for activity
Cricetulus griseus
2.7.8.15
N185A
mutation does not allow plasmid shuffling, indicating that the residue is essential for activity
Cricetulus griseus
2.7.8.15
R123A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
W122R
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
Y256A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
Y256A/F286S
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.15
additional information
-
ALG7 1.5 kb mRNAs appears to be initiated at a start site downstream from that used by the 1.9 and 2.2 kb species and located bentween two in-frame ATG codons. Translation of these transcripts in vitro provides supporting evidence that the 1.5 and 1.9 kb transcripts are translationally competent, giving rise to related protein isoforms with different lengths of their NH2-terminal domains. The 1.9 kb mRNA serves in the synthesis of 36000 Da and 24000 Da species, as well as a low abundance 32000 Da protein. The 1.5 kb transcript gives rise to a translation product of 32000 Da
Cricetulus griseus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Cricetulus griseus
the enzyme is encoded by ALG7, whose expression affects the extent of N-glycosylation and secretion of proteins
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Cricetulus griseus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
brain
-
Cricetulus griseus
-
2.7.8.15
CHO cell
-
Cricetulus griseus
-
2.7.8.15
heart
-
Cricetulus griseus
-
2.7.8.15
kidney
-
Cricetulus griseus
-
2.7.8.15
lung
-
Cricetulus griseus
-
2.7.8.15
spleen
-
Cricetulus griseus
-
2.7.8.15
submandibular gland
-
Cricetulus griseus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645359
Cricetulus griseus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645359
Cricetulus griseus
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
the enzyme is encoded by ALG7, whose expression affects the extent of N-glycosylation and secretion of proteins
645359
Cricetulus griseus
?
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
2.7.8.15
A263D
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
D252A
mutation does not allow plasmid shuffling, indicating that the residues is essential for activity
Cricetulus griseus
2.7.8.15
D252A/F254I
mutation does not allow plasmid shuffling, indicating that the residues are essential for activity
Cricetulus griseus
2.7.8.15
D252A/L102F
mutation does not allow plasmid shuffling, indicating that the residues are essential for activity
Cricetulus griseus
2.7.8.15
F249A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
F257A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
K125A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
N182A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
N182A/I186M
mutation does not allow plasmid shuffling, indicating that the residues are essential for activity
Cricetulus griseus
2.7.8.15
N182A/W122R
mutation does not allow plasmid shuffling, indicating that the residues are essential for activity
Cricetulus griseus
2.7.8.15
N185A
mutation does not allow plasmid shuffling, indicating that the residue is essential for activity
Cricetulus griseus
2.7.8.15
R123A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
W122R
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
Y256A
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
2.7.8.15
Y256A/F286S
mutation allows plasmid shuffling, indicating that the residue is not essential for activity
Cricetulus griseus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.15
additional information
-
ALG7 1.5 kb mRNAs appears to be initiated at a start site downstream from that used by the 1.9 and 2.2 kb species and located bentween two in-frame ATG codons. Translation of these transcripts in vitro provides supporting evidence that the 1.5 and 1.9 kb transcripts are translationally competent, giving rise to related protein isoforms with different lengths of their NH2-terminal domains. The 1.9 kb mRNA serves in the synthesis of 36000 Da and 24000 Da species, as well as a low abundance 32000 Da protein. The 1.5 kb transcript gives rise to a translation product of 32000 Da
Cricetulus griseus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Cricetulus griseus
the enzyme is encoded by ALG7, whose expression affects the extent of N-glycosylation and secretion of proteins
?
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
brain
-
Cricetulus griseus
-
2.7.8.15
CHO cell
-
Cricetulus griseus
-
2.7.8.15
heart
-
Cricetulus griseus
-
2.7.8.15
kidney
-
Cricetulus griseus
-
2.7.8.15
lung
-
Cricetulus griseus
-
2.7.8.15
spleen
-
Cricetulus griseus
-
2.7.8.15
submandibular gland
-
Cricetulus griseus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645359
Cricetulus griseus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645359
Cricetulus griseus
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
the enzyme is encoded by ALG7, whose expression affects the extent of N-glycosylation and secretion of proteins
645359
Cricetulus griseus
?
-
-
-
?