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Literature summary extracted from

  • Mota, O.M.; Huang, G.T.; Kukuruzinska, M.A.
    Developmental regulation and tissue-specific expression of hamster dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT) (1994), Biochem. Biophys. Res. Commun., 204, 284-291.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.15 A263D mutation allows plasmid shuffling, indicating that the residue is not essential for activity Cricetulus griseus
2.7.8.15 D252A mutation does not allow plasmid shuffling, indicating that the residues is essential for activity Cricetulus griseus
2.7.8.15 D252A/F254I mutation does not allow plasmid shuffling, indicating that the residues are essential for activity Cricetulus griseus
2.7.8.15 D252A/L102F mutation does not allow plasmid shuffling, indicating that the residues are essential for activity Cricetulus griseus
2.7.8.15 F249A mutation allows plasmid shuffling, indicating that the residue is not essential for activity Cricetulus griseus
2.7.8.15 F257A mutation allows plasmid shuffling, indicating that the residue is not essential for activity Cricetulus griseus
2.7.8.15 K125A mutation allows plasmid shuffling, indicating that the residue is not essential for activity Cricetulus griseus
2.7.8.15 N182A mutation allows plasmid shuffling, indicating that the residue is not essential for activity Cricetulus griseus
2.7.8.15 N182A/I186M mutation does not allow plasmid shuffling, indicating that the residues are essential for activity Cricetulus griseus
2.7.8.15 N182A/W122R mutation does not allow plasmid shuffling, indicating that the residues are essential for activity Cricetulus griseus
2.7.8.15 N185A mutation does not allow plasmid shuffling, indicating that the residue is essential for activity Cricetulus griseus
2.7.8.15 R123A mutation allows plasmid shuffling, indicating that the residue is not essential for activity Cricetulus griseus
2.7.8.15 W122R mutation allows plasmid shuffling, indicating that the residue is not essential for activity Cricetulus griseus
2.7.8.15 Y256A mutation allows plasmid shuffling, indicating that the residue is not essential for activity Cricetulus griseus
2.7.8.15 Y256A/F286S mutation allows plasmid shuffling, indicating that the residue is not essential for activity Cricetulus griseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.15 additional information
-
 ALG7 1.5 kb mRNAs appears to be initiated at a start site downstream from that used by the 1.9 and 2.2 kb species and located bentween two in-frame ATG codons. Translation of these transcripts in vitro provides supporting evidence that the 1.5 and 1.9 kb transcripts are translationally competent, giving rise to related protein isoforms with different lengths of their NH2-terminal domains. The 1.9 kb mRNA serves in the synthesis of 36000 Da and 24000 Da species, as well as a low abundance 32000 Da protein. The 1.5 kb transcript gives rise to a translation product of 32000 Da Cricetulus griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.15 UDP-N-acetyl-D-glucosamine + dolichyl phosphate Cricetulus griseus the enzyme is encoded by ALG7, whose expression affects the extent of N-glycosylation and secretion of proteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.15 Cricetulus griseus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.15 brain
-
 Cricetulus griseus
-
2.7.8.15 CHO cell
-
 Cricetulus griseus
-
2.7.8.15 heart
-
 Cricetulus griseus
-
2.7.8.15 kidney
-
 Cricetulus griseus
-
2.7.8.15 lung
-
 Cricetulus griseus
-
2.7.8.15 spleen
-
 Cricetulus griseus
-
2.7.8.15 submandibular gland
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.15 UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
 Cricetulus griseus UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?
2.7.8.15 UDP-N-acetyl-D-glucosamine + dolichyl phosphate the enzyme is encoded by ALG7, whose expression affects the extent of N-glycosylation and secretion of proteins Cricetulus griseus ?
-
 ?