BRENDA - Enzyme Database

Diminished activity of the first N-glycosylation enzyme, dolichol-P-dependent N-acetylglucosamine-1-P transferase (GPT), gives rise to mutant phenotypes in yeast

Kukuruzinska, M.A.; Lennon, K.; Biochim. Biophys. Acta 1247, 51-59 (1995)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Saccharomyces cerevisiae
the enzyme initiates the dolichol pathway of protein N-glycosylation. A 2.5- to 4fold reduction in enzyme activity gives rise to distinct phenotypes, whose severity is inversely related to the level of enzyme activity. These phenotypes include hypersensitivity to tunicamycin, enlarged cell size, extensive aggregation, lack of typical stationary arrest and defective spore germination
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645358
Saccharomyces cerevisiae
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645358
Saccharomyces cerevisiae
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
the enzyme initiates the dolichol pathway of protein N-glycosylation. A 2.5- to 4fold reduction in enzyme activity gives rise to distinct phenotypes, whose severity is inversely related to the level of enzyme activity. These phenotypes include hypersensitivity to tunicamycin, enlarged cell size, extensive aggregation, lack of typical stationary arrest and defective spore germination
645358
Saccharomyces cerevisiae
?
-
-
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Saccharomyces cerevisiae
the enzyme initiates the dolichol pathway of protein N-glycosylation. A 2.5- to 4fold reduction in enzyme activity gives rise to distinct phenotypes, whose severity is inversely related to the level of enzyme activity. These phenotypes include hypersensitivity to tunicamycin, enlarged cell size, extensive aggregation, lack of typical stationary arrest and defective spore germination
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645358
Saccharomyces cerevisiae
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645358
Saccharomyces cerevisiae
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
the enzyme initiates the dolichol pathway of protein N-glycosylation. A 2.5- to 4fold reduction in enzyme activity gives rise to distinct phenotypes, whose severity is inversely related to the level of enzyme activity. These phenotypes include hypersensitivity to tunicamycin, enlarged cell size, extensive aggregation, lack of typical stationary arrest and defective spore germination
645358
Saccharomyces cerevisiae
?
-
-
-
-