BRENDA - Enzyme Database

UDP-N-acetylglucosamine:dolichyl-phosphate N-acetylglucosamine-1-phosphate transferase is amplified in tunicamycin-resistant soybean cells

Zeng, Y.; Elbein, A.D.; Eur. J. Biochem. 233, 458-466 (1995)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
ATP
0.01-0.025 mM, stimulates activity of tunicamycin-resistant cells about 20-30%
Glycine max
2.7.8.15
phosphatidylglycerol
activity is slightly enhanced
Glycine max
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
GDP-mannose
slight
Glycine max
2.7.8.15
GTP
0.4 mM, 25-30% inhibition, enzyme from tunicamycin-resistant cells, no inhibition of wild-type enzyme up to 0.4 mM
Glycine max
2.7.8.15
PCMB
the enzyme from tunicamycin-resistant cells is equally sensitive to tunicamycin as the wilde-type enzyme
Glycine max
2.7.8.15
UDP-GlcNAc
strong inhibition
Glycine max
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.15
39000
-
x * 39000, SDS-PAGE
Glycine max
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Glycine max
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
cell culture
the tunicamycin resistant cells have a greater than 40fold increase in the activity of the enzyme UDP-GlcNAc:dolichyl-P GlcNAc1P transferase. Increase in enzyme activity is due to an increased production of the enzyme
Glycine max
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645356
Glycine max
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645356
Glycine max
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.15
?
x * 39000, SDS-PAGE
Glycine max
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.15
35
-
the enzyme from tunicamycin-resistant cells is significantly less stable than the wild-type enzyme
Glycine max
2.7.8.15
40
-
the enzyme from tunicamycin-resistant cells is significantly less stable than the wild-type enzyme
Glycine max
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
ATP
0.01-0.025 mM, stimulates activity of tunicamycin-resistant cells about 20-30%
Glycine max
2.7.8.15
phosphatidylglycerol
activity is slightly enhanced
Glycine max
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
GDP-mannose
slight
Glycine max
2.7.8.15
GTP
0.4 mM, 25-30% inhibition, enzyme from tunicamycin-resistant cells, no inhibition of wild-type enzyme up to 0.4 mM
Glycine max
2.7.8.15
PCMB
the enzyme from tunicamycin-resistant cells is equally sensitive to tunicamycin as the wilde-type enzyme
Glycine max
2.7.8.15
UDP-GlcNAc
strong inhibition
Glycine max
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.15
39000
-
x * 39000, SDS-PAGE
Glycine max
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
cell culture
the tunicamycin resistant cells have a greater than 40fold increase in the activity of the enzyme UDP-GlcNAc:dolichyl-P GlcNAc1P transferase. Increase in enzyme activity is due to an increased production of the enzyme
Glycine max
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645356
Glycine max
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645356
Glycine max
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.15
?
x * 39000, SDS-PAGE
Glycine max
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.15
35
-
the enzyme from tunicamycin-resistant cells is significantly less stable than the wild-type enzyme
Glycine max
2.7.8.15
40
-
the enzyme from tunicamycin-resistant cells is significantly less stable than the wild-type enzyme
Glycine max