BRENDA - Enzyme Database

Specific lipids enhance the activity of UDP-GlcNAc: dolichol phosphate GlcNAc-1-phosphate transferase in rat liver endoplasmic reticulum membrane vesicles

Chandra, N.C.; Doody, M.B.; Bretthauer, R.K.; Arch. Biochem. Biophys. 290, 345-354 (1991)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
cardiolipin
stimulates activity of enzyme in endoplasmic membrane vesicles, due to better solubilization of the dolichol phosphate
Rattus norvegicus
2.7.8.15
monogalactosyldiglyceride
stimulates activity of enzyme in endoplasmic membrane vesicles
Rattus norvegicus
2.7.8.15
phosphatidylethanolamine
stimulates activity of enzyme in endoplasmic membrane vesicles
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
Triton X-100
substrate protects from inhibition
Rattus norvegicus
2.7.8.15
tunicamycin
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
additional information
-
additional information
Km-value for dolichyl phosphate: 0.005 mg/0.1 ml
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
endoplasmic reticulum
-
Rattus norvegicus
5783
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645355
Rattus norvegicus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645355
Rattus norvegicus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.15
40
-
with 0.5% Triton X-100 in the assay mixture
Rattus norvegicus
2.7.8.15
60
-
with either dioleoylphosphatidylethanolamine or cardiolipin in the assay mixture
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
cardiolipin
stimulates activity of enzyme in endoplasmic membrane vesicles, due to better solubilization of the dolichol phosphate
Rattus norvegicus
2.7.8.15
monogalactosyldiglyceride
stimulates activity of enzyme in endoplasmic membrane vesicles
Rattus norvegicus
2.7.8.15
phosphatidylethanolamine
stimulates activity of enzyme in endoplasmic membrane vesicles
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
Triton X-100
substrate protects from inhibition
Rattus norvegicus
2.7.8.15
tunicamycin
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
additional information
-
additional information
Km-value for dolichyl phosphate: 0.005 mg/0.1 ml
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
endoplasmic reticulum
-
Rattus norvegicus
5783
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
liver
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645355
Rattus norvegicus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645355
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.15
40
-
with 0.5% Triton X-100 in the assay mixture
Rattus norvegicus
2.7.8.15
60
-
with either dioleoylphosphatidylethanolamine or cardiolipin in the assay mixture
Rattus norvegicus