BRENDA - Enzyme Database

Properties of solubilized UDP-GlcNAc:dolichyl phosphate-GlcNAc-1-P-transferase from soybean cultured cells

Kaushal, G.P.; Elbein, A.D.; Plant Physiol. 82, 748-752 (1986)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
phosphatidylglycerol
2- to 3fold maximal stimulation at 0.06 mg/ml
Glycine max
2.7.8.15
phosphatidylinositol
stimulates
Glycine max
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
Amphomycin
-
Glycine max
2.7.8.15
Ca2+
above 2 mM
Glycine max
2.7.8.15
Diumycin
-
Glycine max
2.7.8.15
GDP-mannose
-
Glycine max
2.7.8.15
Mn2+
above 2 mM
Glycine max
2.7.8.15
phosphatidylcholine
-
Glycine max
2.7.8.15
phosphatidylethanolamine
-
Glycine max
2.7.8.15
phosphatidylserine
-
Glycine max
2.7.8.15
Showdomycin
-
Glycine max
2.7.8.15
tunicamycin
-
Glycine max
2.7.8.15
UDP-glucose
-
Glycine max
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.00042
-
UDP-GlcNAc
pH 7.5, 37C
Glycine max
2.7.8.15
0.0062
-
dolichyl phosphate
pH 7.5, 37C
Glycine max
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
microsome
-
Glycine max
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Ca2+
1-2 mM, weak stimulation
Glycine max
2.7.8.15
Mg2+
10 mM required for optimal activity
Glycine max
2.7.8.15
Mn2+
1-2 mM, weak stimulation
Glycine max
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Glycine max
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
cell culture
-
Glycine max
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645354
Glycine max
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645354
Glycine max
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.2
7.6
-
Glycine max
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
phosphatidylglycerol
2- to 3fold maximal stimulation at 0.06 mg/ml
Glycine max
2.7.8.15
phosphatidylinositol
stimulates
Glycine max
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
Amphomycin
-
Glycine max
2.7.8.15
Ca2+
above 2 mM
Glycine max
2.7.8.15
Diumycin
-
Glycine max
2.7.8.15
GDP-mannose
-
Glycine max
2.7.8.15
Mn2+
above 2 mM
Glycine max
2.7.8.15
phosphatidylcholine
-
Glycine max
2.7.8.15
phosphatidylethanolamine
-
Glycine max
2.7.8.15
phosphatidylserine
-
Glycine max
2.7.8.15
Showdomycin
-
Glycine max
2.7.8.15
tunicamycin
-
Glycine max
2.7.8.15
UDP-glucose
-
Glycine max
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.00042
-
UDP-GlcNAc
pH 7.5, 37C
Glycine max
2.7.8.15
0.0062
-
dolichyl phosphate
pH 7.5, 37C
Glycine max
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
microsome
-
Glycine max
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Ca2+
1-2 mM, weak stimulation
Glycine max
2.7.8.15
Mg2+
10 mM required for optimal activity
Glycine max
2.7.8.15
Mn2+
1-2 mM, weak stimulation
Glycine max
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
cell culture
-
Glycine max
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645354
Glycine max
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645354
Glycine max
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.2
7.6
-
Glycine max