BRENDA - Enzyme Database

Mechanism of action of tunicamycin on the UDP-GlcNAc:dolichyl-phosphate Glc-NAc-1-phosphate transferase

Heifetz, A.; Keenan, R.W.; Elbein, A.D.; Biochemistry 18, 2186-2192 (1979)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
phosphatidylcholine
0.001 mM/ml, 30-40% inhibition
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
tunicamycin
0.00005-0.0001 mg/ml, 50% inhibition, noncompetitive with respect to UDP-GlcNAc or dolichyl phosphate, both directions
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.0002
-
UDP-GlcNAc
37C
Sus scrofa
2.7.8.15
0.0007
-
dolichyl phosphate
37C
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
microsome
-
Sus scrofa
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Sus scrofa
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
aorta
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645353
Sus scrofa
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645353
Sus scrofa
?
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645353
Sus scrofa
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645353
Sus scrofa
r
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
phosphatidylcholine
0.001 mM/ml, 30-40% inhibition
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
tunicamycin
0.00005-0.0001 mg/ml, 50% inhibition, noncompetitive with respect to UDP-GlcNAc or dolichyl phosphate, both directions
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.0002
-
UDP-GlcNAc
37C
Sus scrofa
2.7.8.15
0.0007
-
dolichyl phosphate
37C
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
microsome
-
Sus scrofa
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
aorta
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645353
Sus scrofa
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645353
Sus scrofa
?
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645353
Sus scrofa
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645353
Sus scrofa
r