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Literature summary extracted from

  • Kaushal, G.P.; Elbein, A.D.
    Purification and properties of UDP-GlcNAc:dolichyl-phosphate GlcNAc-1-phosphate transferase. Activation and inhibition of the enzyme (1985), J. Biol. Chem., 260, 16303-16309.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.15 Dolichyl-phosphoryl-mannose stimulates Sus scrofa
2.7.8.15 GDP-mannose stimulates by protecting the substrate UDP-GlcNAc from degradation Sus scrofa
2.7.8.15 phosphatidylglycerol solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine Sus scrofa
2.7.8.15 phosphatidylinositol solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine Sus scrofa
2.7.8.15 phosphatidylserine solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine Sus scrofa
2.7.8.15 Phospholipid solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.15 NEM protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate and Mn2+ are added before addition of the sulfhydryl reagent Sus scrofa
2.7.8.15 PCMB protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate anf Mn2+ are added before addition of the sulfhydryl reagent Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.15 0.0001
-
 UDP-GlcNAc pH 7.5, 37°C Sus scrofa
2.7.8.15 0.001
-
 dolichyl phosphate pH 7.5, 37°C Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.15 microsome
-
 Sus scrofa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.15 Ca2+ less effective than Mn2+ or Mg2+ Sus scrofa
2.7.8.15 Mg2+ either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity Sus scrofa
2.7.8.15 Mn2+ either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.15 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.15 partial Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.15 aorta
-
 Sus scrofa
-

Storage Stability

EC Number Storage Stability Organism
2.7.8.15 0-4°C, 20% glycerol, 0.02 mg phosphatidylglycerol/mg of protein, less than 20% loss of activity after 6 days. In absence of both glycerol and phosphatidylglycerol the enzyme is quite unstable and loses most of its activity within 24 h Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.15 UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
 Sus scrofa UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.15 7.4 7.6
-
 Sus scrofa