BRENDA - Enzyme Database

Purification and properties of UDP-GlcNAc:dolichyl-phosphate GlcNAc-1-phosphate transferase. Activation and inhibition of the enzyme

Kaushal, G.P.; Elbein, A.D.; J. Biol. Chem. 260, 16303-16309 (1985)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
Dolichyl-phosphoryl-mannose
stimulates
Sus scrofa
2.7.8.15
GDP-mannose
stimulates by protecting the substrate UDP-GlcNAc from degradation
Sus scrofa
2.7.8.15
phosphatidylglycerol
solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine
Sus scrofa
2.7.8.15
phosphatidylinositol
solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine
Sus scrofa
2.7.8.15
phosphatidylserine
solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine
Sus scrofa
2.7.8.15
Phospholipid
solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
NEM
protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate and Mn2+ are added before addition of the sulfhydryl reagent
Sus scrofa
2.7.8.15
PCMB
protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate anf Mn2+ are added before addition of the sulfhydryl reagent
Sus scrofa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.0001
-
UDP-GlcNAc
pH 7.5, 37°C
Sus scrofa
2.7.8.15
0.001
-
dolichyl phosphate
pH 7.5, 37°C
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
microsome
-
Sus scrofa
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Ca2+
less effective than Mn2+ or Mg2+
Sus scrofa
2.7.8.15
Mg2+
either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity
Sus scrofa
2.7.8.15
Mn2+
either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.8.15
partial
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
aorta
-
Sus scrofa
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.8.15
0-4°C, 20% glycerol, 0.02 mg phosphatidylglycerol/mg of protein, less than 20% loss of activity after 6 days. In absence of both glycerol and phosphatidylglycerol the enzyme is quite unstable and loses most of its activity within 24 h
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645351
Sus scrofa
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645351
Sus scrofa
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.4
7.6
-
Sus scrofa
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
Dolichyl-phosphoryl-mannose
stimulates
Sus scrofa
2.7.8.15
GDP-mannose
stimulates by protecting the substrate UDP-GlcNAc from degradation
Sus scrofa
2.7.8.15
phosphatidylglycerol
solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine
Sus scrofa
2.7.8.15
phosphatidylinositol
solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine
Sus scrofa
2.7.8.15
phosphatidylserine
solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine
Sus scrofa
2.7.8.15
Phospholipid
solubilized enzyme is stimulated by exogenously added phospholipids in order of increasing activity: phosphatidylglycerol, phosphatidylinositol, phosphatidylserine
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
NEM
protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate and Mn2+ are added before addition of the sulfhydryl reagent
Sus scrofa
2.7.8.15
PCMB
protected to the extent of about 50% when all of the substrates, UDP-GlcNAc, dolichyl phosphate anf Mn2+ are added before addition of the sulfhydryl reagent
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.0001
-
UDP-GlcNAc
pH 7.5, 37°C
Sus scrofa
2.7.8.15
0.001
-
dolichyl phosphate
pH 7.5, 37°C
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
microsome
-
Sus scrofa
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Ca2+
less effective than Mn2+ or Mg2+
Sus scrofa
2.7.8.15
Mg2+
either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity
Sus scrofa
2.7.8.15
Mn2+
either Mn2+, 1 mM, or Mg2+, 10 mM, required for optimal activity
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.8.15
partial
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
aorta
-
Sus scrofa
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.8.15
0-4°C, 20% glycerol, 0.02 mg phosphatidylglycerol/mg of protein, less than 20% loss of activity after 6 days. In absence of both glycerol and phosphatidylglycerol the enzyme is quite unstable and loses most of its activity within 24 h
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645351
Sus scrofa
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645351
Sus scrofa
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.4
7.6
-
Sus scrofa