BRENDA - Enzyme Database

Purification and characterization of UDP-N-acetyl-D-glucosamine:dolichol phosphate N-acetyl-D-glucosamine-1-phosphate transferase involved in the biosynthesis of asparagine-linked glycoproteins in the mammary gland

Shailubhai, K.; Dong-Yu, B.; Saxena, E.S.; Vijay, I.K.; J. Biol. Chem. 263, 15964-15972 (1988)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
Dolichyl-phosphoryl-mannose
stimulates
Bos taurus
General Stability
EC Number
General Stability
Organism
2.7.8.15
purified enzyme is resistant to digestion with either endo-beta-N-acetylglucosaminidase H or F
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
Diumycin
-
Bos taurus
2.7.8.15
dolichyl phosphate
at high concentrations
Bos taurus
2.7.8.15
Hg2+
partially reversed by dithiothreitol
Bos taurus
2.7.8.15
iodoacetamide
-
Bos taurus
2.7.8.15
PCMB
-
Bos taurus
2.7.8.15
tunicamycin
-
Bos taurus
2.7.8.15
UDP
-
Bos taurus
2.7.8.15
UDP-glucose
-
Bos taurus
2.7.8.15
UDP-hexanolamine
-
Bos taurus
2.7.8.15
UDP-xylose
-
Bos taurus
2.7.8.15
UMP
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.0045
-
UDP-GlcNAc
pH 7.6, 37°C
Bos taurus
2.7.8.15
0.016
-
dolichyl phosphate
pH 7.6, 37°C
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
microsome
-
Bos taurus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Mg2+
stimulates
Bos taurus
2.7.8.15
Mn2+
stimulates
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.15
330000
360000
large molecular mass of the enzyme can be due to aggregation of the hydrophobic, membrane-derived enzyme rather than resulting from subunit interaction, gel filtration
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Bos taurus
enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Bos taurus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.7.8.15
additional information
the enzyme is not a glycoprotein of the asparagine-linked type
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
mammary gland
lactating
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.15
additional information
-
-
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
2.7.8.15
4°C, solubilized enzyme loses most of its activity within 12 h
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645350
Bos taurus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645350
Bos taurus
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins
645350
Bos taurus
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.8.15
More
results indicate that either 70000 MW band in SDS-PAGE is a precursor form of the enzyme or this polypeptide, representing the native enzyme or its subunit, is proteolyzed to smaller, enzymatically active peptides of 50000 Da and 46000 Da during purification
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.4
7.6
-
Bos taurus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.8.15
Dolichyl-phosphoryl-mannose
stimulates
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.8.15
purified enzyme is resistant to digestion with either endo-beta-N-acetylglucosaminidase H or F
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
Diumycin
-
Bos taurus
2.7.8.15
dolichyl phosphate
at high concentrations
Bos taurus
2.7.8.15
Hg2+
partially reversed by dithiothreitol
Bos taurus
2.7.8.15
iodoacetamide
-
Bos taurus
2.7.8.15
PCMB
-
Bos taurus
2.7.8.15
tunicamycin
-
Bos taurus
2.7.8.15
UDP
-
Bos taurus
2.7.8.15
UDP-glucose
-
Bos taurus
2.7.8.15
UDP-hexanolamine
-
Bos taurus
2.7.8.15
UDP-xylose
-
Bos taurus
2.7.8.15
UMP
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.0045
-
UDP-GlcNAc
pH 7.6, 37°C
Bos taurus
2.7.8.15
0.016
-
dolichyl phosphate
pH 7.6, 37°C
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
microsome
-
Bos taurus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Mg2+
stimulates
Bos taurus
2.7.8.15
Mn2+
stimulates
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.8.15
330000
360000
large molecular mass of the enzyme can be due to aggregation of the hydrophobic, membrane-derived enzyme rather than resulting from subunit interaction, gel filtration
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Bos taurus
enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins
?
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.7.8.15
additional information
the enzyme is not a glycoprotein of the asparagine-linked type
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
mammary gland
lactating
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.8.15
additional information
-
-
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.8.15
4°C, solubilized enzyme loses most of its activity within 12 h
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645350
Bos taurus
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645350
Bos taurus
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
enzyme initiates the dolichol cycle for the biosynthesis of asparagine-linked glycoproteins
645350
Bos taurus
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.8.15
More
results indicate that either 70000 MW band in SDS-PAGE is a precursor form of the enzyme or this polypeptide, representing the native enzyme or its subunit, is proteolyzed to smaller, enzymatically active peptides of 50000 Da and 46000 Da during purification
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.4
7.6
-
Bos taurus