BRENDA - Enzyme Database

Properties of a soluble polyprenyl phosphate. UDP-D-N-acetylglucosamine N-acetylglucosamine-1-phosphate transferase

Villemez, C.L.; Carlo, P.L.; J. Biol. Chem. 255, 8174-8178 (1980)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
Diumycin
-
Acanthamoeba castellanii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.0005
-
UDP-GlcNAc
pH 7.4, 30°C
Acanthamoeba castellanii
2.7.8.15
0.0045
-
dolichyl phosphate
pH 7.4, 30°C
Acanthamoeba castellanii
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Mg2+
divalent cation required for dialyzed enzyme, maximal activity in presence of 8-10 mM
Acanthamoeba castellanii
2.7.8.15
Mn2+
divalent cation required for dialyzed enzyme, Mg2+ or Mn2+
Acanthamoeba castellanii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Acanthamoeba castellanii
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
cell culture
-
Acanthamoeba castellanii
-
2.7.8.15
cyst
-
Acanthamoeba castellanii
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.8.15
-20°C or -70°C, stable for 2 weeks
Acanthamoeba castellanii
2.7.8.15
4°C, completely stable for 9-10 days. No significant loss of activity after 63 days in presence of NaN3
Acanthamoeba castellanii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645349
Acanthamoeba castellanii
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645349
Acanthamoeba castellanii
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.15
30
-
-
Acanthamoeba castellanii
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.8.15
25
40
0°C: no activity, 15°C: 11% of maximal activity, 25°C: 73% of maximal activity, 35°C: 96% of maximal activity, 40°C: 57% of maximal activity
Acanthamoeba castellanii
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.15
30
-
1 h, stable
Acanthamoeba castellanii
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.4
7.8
-
Acanthamoeba castellanii
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.8.15
7
8.7
pH 6.0: no activity, pH 7.0: 85% of maximal activity, pH 8.7: 64% of maximal activity
Acanthamoeba castellanii
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
Diumycin
-
Acanthamoeba castellanii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.0005
-
UDP-GlcNAc
pH 7.4, 30°C
Acanthamoeba castellanii
2.7.8.15
0.0045
-
dolichyl phosphate
pH 7.4, 30°C
Acanthamoeba castellanii
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Mg2+
divalent cation required for dialyzed enzyme, maximal activity in presence of 8-10 mM
Acanthamoeba castellanii
2.7.8.15
Mn2+
divalent cation required for dialyzed enzyme, Mg2+ or Mn2+
Acanthamoeba castellanii
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.8.15
cell culture
-
Acanthamoeba castellanii
-
2.7.8.15
cyst
-
Acanthamoeba castellanii
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.8.15
-20°C or -70°C, stable for 2 weeks
Acanthamoeba castellanii
2.7.8.15
4°C, completely stable for 9-10 days. No significant loss of activity after 63 days in presence of NaN3
Acanthamoeba castellanii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645349
Acanthamoeba castellanii
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645349
Acanthamoeba castellanii
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.8.15
30
-
-
Acanthamoeba castellanii
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.8.15
25
40
0°C: no activity, 15°C: 11% of maximal activity, 25°C: 73% of maximal activity, 35°C: 96% of maximal activity, 40°C: 57% of maximal activity
Acanthamoeba castellanii
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.8.15
30
-
1 h, stable
Acanthamoeba castellanii
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.4
7.8
-
Acanthamoeba castellanii
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.8.15
7
8.7
pH 6.0: no activity, pH 7.0: 85% of maximal activity, pH 8.7: 64% of maximal activity
Acanthamoeba castellanii