BRENDA - Enzyme Database

Solubilization and characterization of the initial enzymes of the dolichol pathway from yeast

Sharma, C.B.; Lehle, L.; Tanner, W.; Eur. J. Biochem. 126, 319-325 (1982)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.8.15
a 1:4 dilution leads to a 65% loss of activity
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
tunicamycin
1 mM, complete inhibition
Saccharomyces cerevisiae
2.7.8.15
UDP
1 mM, 55% inhibition
Saccharomyces cerevisiae
2.7.8.15
UMP
1 mM, 45% inhibition
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.001
-
dolichyl phosphate
pH 7.5
Saccharomyces cerevisiae
2.7.8.15
0.015
-
UDP-GlcNAc
pH 7.5
Saccharomyces cerevisiae
2.7.8.15
0.18
-
dolichyl phosphate
pH 7.5
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
membrane
-
Saccharomyces cerevisiae
16020
-
2.7.8.15
microsome
-
Saccharomyces cerevisiae
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Ca2+
divalent metal required, Ca2+ is less effective than Mg2+
Saccharomyces cerevisiae
2.7.8.15
Mg2+
divalent metal required, optimal activity in presence of Mg2+
Saccharomyces cerevisiae
2.7.8.15
Mn2+
divalent metal required, Mn2+ is less effective than Mg2+
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Saccharomyces cerevisiae
first enzyme of dolichol pathway
?
-
-
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Saccharomyces cerevisiae X2180-1A
first enzyme of dolichol pathway
?
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.8.15
Saccharomyces cerevisiae
-
X2180-1A
-
2.7.8.15
Saccharomyces cerevisiae X2180-1A
-
X2180-1A
-
Storage Stability
EC Number
Storage Stability
Organism
2.7.8.15
-20C, solubilized enzyme loses 96% of its activity after 6 days, only 20% loss of activity after 6 days in presence of 0.07 mM UDP-GlcNAc
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645348
Saccharomyces cerevisiae
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645348
Saccharomyces cerevisiae
?
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645348
Saccharomyces cerevisiae X2180-1A
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645348
Saccharomyces cerevisiae X2180-1A
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
first enzyme of dolichol pathway
645348
Saccharomyces cerevisiae
?
-
-
-
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
first enzyme of dolichol pathway
645348
Saccharomyces cerevisiae X2180-1A
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.5
-
-
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.8.15
6.5
8.5
pH 6.5: about 60% of maximal activity, pH 8.5: about 35% of maximal activity
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.8.15
a 1:4 dilution leads to a 65% loss of activity
Saccharomyces cerevisiae
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.8.15
tunicamycin
1 mM, complete inhibition
Saccharomyces cerevisiae
2.7.8.15
UDP
1 mM, 55% inhibition
Saccharomyces cerevisiae
2.7.8.15
UMP
1 mM, 45% inhibition
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.8.15
0.001
-
dolichyl phosphate
pH 7.5
Saccharomyces cerevisiae
2.7.8.15
0.015
-
UDP-GlcNAc
pH 7.5
Saccharomyces cerevisiae
2.7.8.15
0.18
-
dolichyl phosphate
pH 7.5
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.8.15
membrane
-
Saccharomyces cerevisiae
16020
-
2.7.8.15
microsome
-
Saccharomyces cerevisiae
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.8.15
Ca2+
divalent metal required, Ca2+ is less effective than Mg2+
Saccharomyces cerevisiae
2.7.8.15
Mg2+
divalent metal required, optimal activity in presence of Mg2+
Saccharomyces cerevisiae
2.7.8.15
Mn2+
divalent metal required, Mn2+ is less effective than Mg2+
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Saccharomyces cerevisiae
first enzyme of dolichol pathway
?
-
-
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
Saccharomyces cerevisiae X2180-1A
first enzyme of dolichol pathway
?
-
-
?
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.8.15
-20C, solubilized enzyme loses 96% of its activity after 6 days, only 20% loss of activity after 6 days in presence of 0.07 mM UDP-GlcNAc
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645348
Saccharomyces cerevisiae
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645348
Saccharomyces cerevisiae
?
2.7.8.15
UDP-N-acetyl-alpha-D-glucosamine + dolichyl phosphate
-
645348
Saccharomyces cerevisiae X2180-1A
UMP + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
645348
Saccharomyces cerevisiae X2180-1A
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
first enzyme of dolichol pathway
645348
Saccharomyces cerevisiae
?
-
-
-
?
2.7.8.15
UDP-N-acetyl-D-glucosamine + dolichyl phosphate
first enzyme of dolichol pathway
645348
Saccharomyces cerevisiae X2180-1A
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.8.15
7.5
-
-
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.8.15
6.5
8.5
pH 6.5: about 60% of maximal activity, pH 8.5: about 35% of maximal activity
Saccharomyces cerevisiae