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Literature summary extracted from

  • Azzolina, B.A.; Yuan, X.; Anderson, M.S.; El-Sherbeini, M.
    The cell wall and cell division gene cluster in the Mra operon of Pseudomonas aeruginosa: Cloning, production, and purification of active enzymes (2001), Protein Expr. Purif., 21, 393-400.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.8.13 medicine opportunistic pathogen causing infections in patients with burns or neutropenia and involved in respiratory tract infections of cystic fibrosis patients, pathway of peptidoglycan biosynthesis is both essential and unique to bacteria, making it an attractive target for antibiotic research Pseudomonas aeruginosa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.13 cloning of the murE-dd1B region, the cell wall biosynthesis and cell division gene cluster, expressed in Escherichia coli BL21(DE3) Pseudomonas aeruginosa
6.3.2.13 expression in Escherichia coli Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.13 cytoplasm
-
 Pseudomonas aeruginosa 5737
-
2.7.8.13 membrane
-
 Pseudomonas aeruginosa 16020
-
6.3.2.13 cytoplasm
-
 Pseudomonas aeruginosa 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + acceptor Pseudomonas aeruginosa
-
 UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
 r
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate Pseudomonas aeruginosa
-
 ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.13 Pseudomonas aeruginosa
-
-
-
6.3.2.13 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.13
-
 Pseudomonas aeruginosa
6.3.2.13 recombinant MurE, Talon resin Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.13 3.31
-
 recombinant MurE Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + acceptor
-
 Pseudomonas aeruginosa UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
 r
6.3.2.13 ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate
-
 Pseudomonas aeruginosa ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.13 MurE
-
 Pseudomonas aeruginosa