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Literature summary extracted from

  • Geis, A.; Plapp, R.
    Phospho-N-acetylmuramoyl-pentapeptide-transferase of Escherichia coli K12. Properties of the membrane-bound and the extracted and partially purified enzyme (1978), Biochim. Biophys. Acta, 527, 414-424.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.13 Moenomycin activates partially purified enzyme, maximal activation at 1 mg moenomycin/mg protein Escherichia coli
2.7.8.13 Phospholipid phospholipide sensitive to phospholipase necessary for enzymatic activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.13 0.0056
-
 UDP-NAc-muramoyl-pentapeptide pH 7.8, 37°C, crude envelopes Escherichia coli
2.7.8.13 0.037
-
 UDP-NAc-muramoyl-pentapeptide pH 7.8, 37°C, solubilized enzyme Escherichia coli
2.7.8.13 0.067
-
 5'-UMP pH 7.8, 37°C, solubilized enzyme Escherichia coli
2.7.8.13 0.087
-
 5'-UMP pH 7.8, 37°C, crude envelopes Escherichia coli
2.7.8.13 0.087
-
 UDP-MurNAc-pentapeptide pH 7.8, 37°C, at 0.016 mM C55-isoprenylphosphate Escherichia coli
2.7.8.13 0.27
-
 UDP-MurNAc-pentapeptide pH 7.8, 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.13 cell envelope
-
 Escherichia coli 30313
-
2.7.8.13 cytoplasm
-
 Escherichia coli 5737
-
2.7.8.13 membrane
-
 Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.13 Mg2+ absolute requirement, maximun activity at 30-40 mM Escherichia coli
2.7.8.13 Mn2+ can substitute for Mg2+ Escherichia coli
2.7.8.13 additional information Cu2+, Ca2+ and Zn2+ are without effect Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + acceptor Escherichia coli
-
 UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.13 Escherichia coli
-
-
-
2.7.8.13 Escherichia coli
-
 K12 ATCC 23811
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.13 partially Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.13 UDP-MurNAc-pentapeptide + C55-isoprenyl phosphate
-
 Escherichia coli C55-isoprenyl-diphosphate-MurNAc-pentapeptide
-
 r
2.7.8.13 UDP-N-acetylmuramoyl-pentapeptide + [2H]UMP
-
 Escherichia coli UMP + [2H]UDP-NAc-muramoyl-pentapeptide
-
 r
2.7.8.13 UDP-NAc-muramoyl-pentapeptide + acceptor
-
 Escherichia coli UMP + acceptor-phospho-NAc-muramoyl-pentapeptide
-
 r