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Literature summary extracted from

  • Norman, R.A.; McAlister, M.S.; Murray-Rust, J.; Movahedzadeh, F.; Stoker, N.G.; McDonald, N.Q.
    Crystal structure of inositol 1-phosphate synthase from Mycobacterium tuberculosis, a key enzyme in phosphatidylinositol synthesis (2002), Structure, 10, 393-402.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.8.11 medicine enzymes involved in the PI biosynthetic pathway are potential antimycobacterial agents Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.11 tbINO, the coding sequence of ino1 amplified by PCR, cloned into expression vector pET-15b and overproduced in Escherichia coli BL21 (DE3) Mycobacterium tuberculosis
5.5.1.4
-
 Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.8.11 bound to the cofactor NAD+, space group P42(1)2, cell dimensions a = 116.2, b = 116.2, c = 64.5 Mycobacterium tuberculosis
5.5.1.4 hanging-drop vapour-diffusion method, crystal structure of native and selenomethionine-bound enzyme at 1.95 resolution, bound to the cofactor NAD+ Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.5.1.4 Zn2+ enzyme contains Zn2+. The nicotinamide moiety of the NAD+ cofactor is in the syn conformation about the N-glycosidic bond and is held there by coordination with the Zn2+ ion. N233, D235 and D310 provide ligands to a water molecule that coordinates the zinc ion Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + myo-inositol Mycobacterium tuberculosis
-
 CMP + phosphatidyl-1D-myo-inositol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.11 Mycobacterium tuberculosis
-
-
-
5.5.1.4 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.11 recombinant enzyme Mycobacterium tuberculosis
5.5.1.4
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + myo-inositol
-
 Mycobacterium tuberculosis CMP + phosphatidyl-1D-myo-inositol
-
 r
5.5.1.4 D-glucose 6-phosphate key enzyme in inositol biosynthesis, rate-limiting step Mycobacterium tuberculosis 1D-inositol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.4 tbINO
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.8.11 NAD+
-
 Mycobacterium tuberculosis
5.5.1.4 NAD+
-
 Mycobacterium tuberculosis