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Literature summary extracted from
- Phosphatidylinositol synthase from yeast (1997), Biochim. Biophys. Acta, 1348, 173-178.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.8.11 | Triton X-100 | - |
Rattus norvegicus |  |
| 2.7.8.11 | Triton X-100 | reaction is dependent on for maximum activity | Saccharomyces cerevisiae | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.7.8.11 | - |
Rattus norvegicus |
| 2.7.8.11 | disruption of the PIS locus in the genome is lethal | Saccharomyces cerevisiae |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 2.7.8.11 | H114Q | oligonucleotide-mediated site-directed mutagenesis, substitution of amino acid at position 114 from His (CAC) + Gln (CAA) results in a 200fold increase in Km of the enzyme for myo-inositol, making cells auxotrophic for myo-inositol | Saccharomyces cerevisiae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.7.8.11 | 0.07 | - |
CDP-diacylglycerol | ? | Saccharomyces cerevisiae | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.7.8.11 | microsome | - |
Rattus norvegicus | - |
- |
| 2.7.8.11 | microsome | - |
Saccharomyces cerevisiae | - |
- |
| 2.7.8.11 | mitochondrial membrane | outer mitochondrial membrane | Saccharomyces cerevisiae | 31966 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.8.11 | Mg2+ | - |
Rattus norvegicus | |
| 2.7.8.11 | Mg2+ | - |
Saccharomyces cerevisiae | |
| 2.7.8.11 | Mn2+ | - |
Rattus norvegicus | |
| 2.7.8.11 | Mn2+ | - |
Saccharomyces cerevisiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 23610 | - |
primary translate deduced from the encoding gene, PIS | Rattus norvegicus |
| 2.7.8.11 | 24820 | - |
calculated molecular mass from the cloned PI synthase gene | Saccharomyces cerevisiae |
| 2.7.8.11 | 34000 | - |
- |
Rattus norvegicus |
| 2.7.8.11 | 34000 | - |
SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Rattus norvegicus | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Saccharomyces cerevisiae | - |
CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.8.11 | no activity in Escherichia coli | - |
- |
- |
| 2.7.8.11 | Rattus norvegicus | - |
rat | - |
| 2.7.8.11 | Saccharomyces cerevisiae | - |
yeast | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.8.11 | - |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Rattus norvegicus | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Saccharomyces cerevisiae | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.7.8.11 | PI synthase | - |
Saccharomyces cerevisiae |
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