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Literature summary extracted from

  • Parries, G.S.; Hokin-Neaverson, M.
    Phosphatidylinositol synthase from canine pancreas: solubilization by n-octyl glucopyranoside and stabilization by manganese (1984), Biochemistry, 23, 4785-4791.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.8.11 0.018
-
 CDP-diacylglycerol pH 8.5, 37°C Canis lupus familiaris
2.7.8.11 0.76
-
 myo-inositol pH 8.5, 37°C Canis lupus familiaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.11 endoplasmic reticulum
-
 Canis lupus familiaris 5783
-
2.7.8.11 membrane membrane-bound Canis lupus familiaris 16020
-
2.7.8.11 microsome
-
 Canis lupus familiaris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.11 Mg2+
-
 Canis lupus familiaris
2.7.8.11 Mn2+
-
 Canis lupus familiaris
2.7.8.11 additional information replacement of Mn2+ or Mg2+ with 1 mM Co2+ give approximately 10% of maximal activity, 1 mM Zn2+, Ni2+, Cu2+, Ca2+ and Fe2+ does not activate the enzyme Canis lupus familiaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.11 30000
-
 ? * 30000 + ? * 60000, SDS-PAGE Canis lupus familiaris
2.7.8.11 60000
-
 ? * 30000 + ? * 60000, SDS-PAGE Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + myo-inositol Canis lupus familiaris enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol CMP + phosphatidyl-1D-myo-inositol
-
 r
2.7.8.11 CDP-diacylglycerol + myo-inositol Canis lupus familiaris enzyme plays a role in resynthesis of phosphatidylinositol during agonist-stimulated inositol-phospholipid metabolism CMP + phosphatidyl-1D-myo-inositol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.11 Canis lupus familiaris
-
 dog
-
2.7.8.11 Canis lupus familiaris
-
 canine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.11 partially Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.8.11 pancreas
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.11 0.03
-
-
 Canis lupus familiaris
2.7.8.11 0.29
-
-
 Canis lupus familiaris

Storage Stability

EC Number Storage Stability Organism
2.7.8.11 -80°C, no appreciable loss of PI synthase activity detected for up to at least 6 months Canis lupus familiaris
2.7.8.11 4°C, 67% of the activity remains after 3 days Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + myo-inositol
-
 Canis lupus familiaris CMP + phosphatidyl-1D-myo-inositol
-
 r
2.7.8.11 CDP-diacylglycerol + myo-inositol enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol Canis lupus familiaris CMP + phosphatidyl-1D-myo-inositol
-
 r
2.7.8.11 CDP-diacylglycerol + myo-inositol enzyme plays a role in resynthesis of phosphatidylinositol during agonist-stimulated inositol-phospholipid metabolism Canis lupus familiaris CMP + phosphatidyl-1D-myo-inositol
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.8.11 ? ? * 30000 + ? * 60000, SDS-PAGE Canis lupus familiaris

Synonyms

EC Number Synonyms Comment Organism
2.7.8.11 PI synthase
-
 Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.11 6.5 7 pH optimum in reverse direction Canis lupus familiaris
2.7.8.11 8.5 9 optimum in the forward direction Canis lupus familiaris