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Literature summary extracted from

  • Fischl, A.S.; Carman, G.M.
    Phosphatidylinositol biosynthesis in Saccharomyces cerevisiae: purification and properties of microsome-associated phosphatidylinositol synthase (1983), J. Bacteriol., 154, 304-311.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.11 Triton X-100 reaction is dependent on for maximum activity Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.7.8.11 activity is stable for at least 4 cycles of freezing and thawing Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.8.11 Cd2+
-
 Saccharomyces cerevisiae
2.7.8.11 Hg2+
-
 Saccharomyces cerevisiae
2.7.8.11 additional information inactivated by thioreactive reagents Saccharomyces cerevisiae
2.7.8.11 p-chloromercuribenzene sulfonate
-
 Saccharomyces cerevisiae
2.7.8.11 Zn2+
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.11 membrane
-
 Saccharomyces cerevisiae 16020
-
2.7.8.11 microsome
-
 Saccharomyces cerevisiae
-
-
2.7.8.11 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.8.11 Mg2+ either Mg2+ or Mn2+ ions are necessary Saccharomyces cerevisiae
2.7.8.11 Mn2+ absolute requirement, reaction is dependent on for maximum activity Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.11 34000
-
 ? * 34000, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + myo-inositol Saccharomyces cerevisiae formation of membrane phospholipids essential for the growth CMP + phosphatidyl-1D-myo-inositol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.11 Saccharomyces cerevisiae
-
 yeast
-
2.7.8.11 Saccharomyces cerevisiae
-
 wild-type strain S288C(alpha gal2)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.11
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.8.11 0.8
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.8.11 -80°C, purified enzyme is completely stable for at least 6 months Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.11 CDP-diacylglycerol + myo-inositol
-
 Saccharomyces cerevisiae CMP + phosphatidyl-1D-myo-inositol
-
 r
2.7.8.11 CDP-diacylglycerol + myo-inositol formation of membrane phospholipids essential for the growth Saccharomyces cerevisiae CMP + phosphatidyl-1D-myo-inositol
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.8.11 ? ? * 34000, SDS-PAGE Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.7.8.11 PI synthase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.11 35
-
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.8.11 60
-
 reasonably stable up to, essentially 100% stable for at least 10 min at temperatures ranging from 5 to 50°C, inactivation of about 35% at 60°C and 100% at 70°C Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.8.11 8
-
-
 Saccharomyces cerevisiae