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Literature summary extracted from
- Solubilization of microsomal-associated phosphatidylinositol synthase from germinating soybeans (1982), Plant Physiol., 69, 146-149.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.7.8.11 | Triton X-100 | - |
Glycine max |  |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.7.8.11 | stable to at least 2 cycles of freezing and thawing with no loss in enzyme activity | Glycine max |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.7.8.11 | endoplasmic reticulum | - |
Glycine max | 5783 | - |
| 2.7.8.11 | membrane | - |
Glycine max | 16020 | - |
| 2.7.8.11 | microsome | - |
Glycine max | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | Glycine max | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.7.8.11 | Glycine max | - |
soybean | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.7.8.11 | - |
Glycine max |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.7.8.11 | germ | - |
Glycine max | - |
| 2.7.8.11 | seed | - |
Glycine max | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.7.8.11 | -80°C, glycerol stabilizes PI synthase to storage, stable to storage for at least 3 months | Glycine max |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | - |
Glycine max | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
| 2.7.8.11 | CDP-diacylglycerol + myo-inositol | enzyme catalyzes the final step of de novo synthesis of phosphatidylinositol | Glycine max | CMP + phosphatidyl-1D-myo-inositol | - |
r | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.7.8.11 | 30 | - |
thermally inactivated at temperatures above, thermally unstable when incubated for 20 min, negligible activity present at 45°C | Glycine max |
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