Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Potvin, B.; Raju, T.S.; Stanley, P.
    Lec32 is a new mutation in Chinese hamster ovary cells that essentially abrogates CMP-N-acetylneuraminic acid synthetase activity (1995), J. Biol. Chem., 270, 30415-30421.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.43 CTP above 5 mM Cricetulus griseus
2.7.7.43 Cu2+ inhibition in presence of Mg2+ Cricetulus griseus
2.7.7.43 NEM
-
 Cricetulus griseus
2.7.7.43 Zn2+ inhibits in presence of Mg2+ Cricetulus griseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.34
-
 N-acetylneuraminate pH 9.0, 37°C Cricetulus griseus
2.7.7.43 1.3
-
 CTP pH 9.0, 37°C Cricetulus griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Fe2+ 10% of the activation with Mg2+ Cricetulus griseus
2.7.7.43 Mg2+ maximal activity at 20 mM Cricetulus griseus
2.7.7.43 Mn2+ 10% of the activity with Mg2+ Cricetulus griseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate Cricetulus griseus the lec32 mutation reduces CMP-NeuAc synthetase activity to undetectable levels and reduces Neu-Ac on glycoproteins and glycolipids by 95% diphosphate + CMP-N-acylneuraminate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Cricetulus griseus
-
 wild-type and glycosylation mutant LEC29.Lec32, the mutation resides in either the structural gene encoding CMP-NeuAc synthetase or in a gene that regulates the prpduction of the active enzyme
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.43 CHO cell
-
 Cricetulus griseus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate
-
 Cricetulus griseus diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate the lec32 mutation reduces CMP-NeuAc synthetase activity to undetectable levels and reduces Neu-Ac on glycoproteins and glycolipids by 95% Cricetulus griseus diphosphate + CMP-N-acylneuraminate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.43 37
-
-
 Cricetulus griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.43 7
-
 reaction with Mn2+ Cricetulus griseus
2.7.7.43 8.5 9 activity in presence of Mg2+ Cricetulus griseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.43 7.5 9.5 pH 7.5: about 20% of maximal activity, pH 8.0: about 90% of maximal activity, pH 9.5: about 65% of maximal activity Cricetulus griseus