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Literature summary extracted from

  • Schauer, R.; Haverkamp, J.; Ehrlich, K.
    Isolation and characterization of acylneuraminate cytidylyltransferase from frog liver (1980), Hoppe-Seyler's Z. Physiol. Chem., 361, 641-648.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.43 stable in the frozen state, each cycle of freezing and thawing leads to a 15% decrease in activity Pelophylax lessonae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.43 CMP noncompetitive Pelophylax lessonae
2.7.7.43 Cu2+
-
 Pelophylax lessonae
2.7.7.43 EDTA
-
 Pelophylax lessonae
2.7.7.43 Hg2+
-
 Pelophylax lessonae
2.7.7.43 iodoacetate
-
 Pelophylax lessonae
2.7.7.43 PCMB
-
 Pelophylax lessonae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.6
-
 CTP pH 9.0, 37°C Pelophylax lessonae
2.7.7.43 1.6
-
 N-acetylneuraminate pH 9.0, 37°C Pelophylax lessonae
2.7.7.43 2.3
-
 N-glycolylneuraminate pH 9.0, 37°C Pelophylax lessonae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.43 soluble
-
 Pelophylax lessonae
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Ca2+ maximal activity in presence of 50 mM Mg2+ or Ca2+ Pelophylax lessonae
2.7.7.43 Mg2+ maximal activity in presence of 50 mM Mg2+ or Ca2+ Pelophylax lessonae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.43 162000
-
 gel filtration Pelophylax lessonae
2.7.7.43 164000
-
 1 * 164000, SDS-PAGE Pelophylax lessonae

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Pelophylax lessonae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43
-
 Pelophylax lessonae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.43 0.542
-
-
 Pelophylax lessonae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate
-
 Pelophylax lessonae diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-glycolylneuraminate
-
 Pelophylax lessonae diphosphate + CMP-N-glycolylneuraminate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.43 monomer 1 * 164000, SDS-PAGE Pelophylax lessonae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.43 37
-
-
 Pelophylax lessonae
2.7.7.43 38
-
-
 Pelophylax lessonae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.43 33 42 50% of maximal activity at 33°C and at 42°C Pelophylax lessonae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.43 9
-
-
 Pelophylax lessonae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.43 0.5
-
 CMP pH 9.0, 37°C Pelophylax lessonae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.43 Pelophylax lessonae isoelectric focusing
-
 5.9