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Literature summary extracted from

  • Vann, W.F.; Silver, R.P.; Abeijon, C.; Chang, K.; Aaronson, W.; Sutton, A.; Finn, C.W.; Lindner, W.; Kotsatos, M.
    Purification, properties, and genetic location of Escherichia coli cytidine 5-monophosphate N-acetylneuraminic acid synthetase (1987), J. Biol. Chem., 262, 17556-17562.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.43 5-Mercuri-CTP
-
 Escherichia coli
2.7.7.43 EDTA
-
 Escherichia coli
2.7.7.43 periodate-oxidized CTP
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.31
-
 CTP pH 9.0, 37°C Escherichia coli
2.7.7.43 4
-
 N-acetylneuraminate pH 9.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Mg2+ Mg2+ or Mn2+ required, optimal activity with 20-40 mM Mg2+ Escherichia coli
2.7.7.43 Mn2+ Mg2+ or Mn2+ required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.43 50000
-
 x * 50000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Escherichia coli
-
 O18:K1
-
2.7.7.43 Escherichia coli O18:K1
-
 O18:K1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.43 2.1
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.43 4°C, above pH 7.0, stable for several weeks Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CDP + N-acetylneuraminate
-
 Escherichia coli phosphate + CMP-N-acetylneuraminate
-
 ?
2.7.7.43 CDP + N-acetylneuraminate
-
 Escherichia coli O18:K1 phosphate + CMP-N-acetylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Escherichia coli diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Escherichia coli O18:K1 diphosphate + CMP-N-acylneuraminate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.43 ? x * 50000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.43 9 10
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.43 7
-
 above, 4°C, stable for 4 weeks Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.7.43 Escherichia coli isoelectric focusing
-
 5