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Literature summary extracted from

  • Haft, R.F.; Wessels, M.R.
    Characterization of CMP-N-acetylneuraminic acid synthetase of group B streptococci (1994), J. Bacteriol., 176, 7372-7374.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.43 dithiothreitol activates but enzyme is not dependent on Streptococcus sp.

General Stability

EC Number General Stability Organism
2.7.7.43 the enzyme tolerates flash freezing and lyophilization Streptococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 1.4
-
 CTP
-
 Streptococcus sp.
2.7.7.43 7.6
-
 N-acetylneuraminate
-
 Streptococcus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Ca2+ no effect Streptococcus sp.
2.7.7.43 Mg2+ required Streptococcus sp.
2.7.7.43 Mg2+ maximal activity at 20 mM or above Streptococcus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate Streptococcus sp. the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate Streptococcus sp. Ib the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide diphosphate + CMP-N-acylneuraminate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Streptococcus sp.
-
 group B, high-producing type Ib strain
-
2.7.7.43 Streptococcus sp. Ib
-
 group B, high-producing type Ib strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43
-
 Streptococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.43 0.354
-
-
 Streptococcus sp.

Storage Stability

EC Number Storage Stability Organism
2.7.7.43 -80°C, completely stable for 8 months Streptococcus sp.
2.7.7.43 4°C, pH 7.6, 83% of the original activity is recovered after 4 weeks Streptococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate
-
 Streptococcus sp. diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide Streptococcus sp. diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Streptococcus sp. Ib diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate the enzyme is essential in capsular polysaccharide biosynthesis, activates N-acetylneuraminate for transfer to the nascent capsular polysaccharide Streptococcus sp. Ib diphosphate + CMP-N-acylneuraminate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.43 25 37
-
 Streptococcus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.43 25 42 4°C: activity is undetectable, 25-37°C: maximal activity, 42°C: 12% of maximal activity Streptococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.43 8.3 9.4
-
 Streptococcus sp.