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Literature summary extracted from

  • Kean, E.L.; Roseman, S.
    The sialic acids. X. Purification and properties of cytidine 5-monophosphosialic acid synthetase (1966), J. Biol. Chem., 241, 5643-5650.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.7.43 not stable to freezing and thawing, addition of substrates partially stabilizes the purified enzyme to freezing and thawing Sus scrofa
2.7.7.43 not stable to lyophilization Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.43 Ca2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
2.7.7.43 Cd2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
2.7.7.43 Co2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
2.7.7.43 Cu2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
2.7.7.43 Mn2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa
2.7.7.43 Zn2+ inhibits when incubated in presence of Mg2+ at the same concentration Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.43 0.6
-
 CTP pH 9.0, 37°C Sus scrofa
2.7.7.43 0.8
-
 N-acylneuraminate pH 9.0, 37°C Sus scrofa
2.7.7.43 2.3
-
 N-glycolylneuraminate pH 9.0, 37°C Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.43 Ca2+ divalent cation required, at 20 mM 20% as effective as Mg2+ Sus scrofa
2.7.7.43 Co2+ divalent cation required, at 20 mM 10% as effective as Mg2+ Sus scrofa
2.7.7.43 Fe2+ divalent cation required, at 20 mM 10% as effective as Mg2+ Sus scrofa
2.7.7.43 Mg2+
-
 Homo sapiens
2.7.7.43 Mg2+ divalent cation required, maximal stimulation at 20 mM Sus scrofa
2.7.7.43 Mn2+ divalent cation required, at 20 mM 20% as effective as Mg2+ Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.43 Bos taurus
-
-
-
2.7.7.43 Homo sapiens
-
-
-
2.7.7.43 Ovis aries
-
-
-
2.7.7.43 Sus scrofa
-
 hog
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.43
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.43 brain
-
 Sus scrofa
-
2.7.7.43 epididymis
-
 Sus scrofa
-
2.7.7.43 kidney
-
 Sus scrofa
-
2.7.7.43 liver
-
 Sus scrofa
-
2.7.7.43 lung
-
 Sus scrofa
-
2.7.7.43 mammary gland
-
 Sus scrofa
-
2.7.7.43 spleen
-
 Sus scrofa
-
2.7.7.43 submaxillary gland
-
 Homo sapiens
-
2.7.7.43 submaxillary gland
-
 Sus scrofa
-
2.7.7.43 submaxillary gland
-
 Bos taurus
-
2.7.7.43 submaxillary gland
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.43 0.83
-
 at pH 7.6 Sus scrofa
2.7.7.43 1.58 3.17 at pH 9.0 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
2.7.7.43 4°C, stable for 3 weeks Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.43 CTP + N-acylneuraminate
-
 Homo sapiens diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Sus scrofa diphosphate + CMP-N-acylneuraminate
-
 r
2.7.7.43 CTP + N-acylneuraminate
-
 Bos taurus diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-acylneuraminate
-
 Ovis aries diphosphate + CMP-N-acylneuraminate
-
 ?
2.7.7.43 CTP + N-glycolylneuraminate
-
 Sus scrofa diphosphate + CMP-N-glycolylneuraminate
-
 r
2.7.7.43 CTP + N-glycolylneuraminate
-
 Bos taurus diphosphate + CMP-N-glycolylneuraminate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.43 22
-
 not stable at room temperature for 12 h Sus scrofa
2.7.7.43 37
-
 reaction rate is proportional to enzyme concentration and constant for at least 45 min. At high enzyme concentrations the reaction rate decreases 10-15% between 45 and 60 min Sus scrofa