EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.405 | expression in Escherichia coli | Haemophilus influenzae |
2.7.7.40 | - |
Haemophilus influenzae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.405 | K386A | mutant impaired for reduction of D-ribulose. Equimolar mixtures of mutants K386A and R18A are efficient for bifunctional catalysis | Haemophilus influenzae |
1.1.1.405 | R18A | mutant impaired for cytidylyl transfer. Equimolar mixtures of mutants K386A and R18A are efficient for bifunctional catalysis | Haemophilus influenzae |
2.7.7.40 | K368A | kinetic values similar to wild-type enzyme | Haemophilus influenzae |
2.7.7.40 | R18A | reduced enzyme activity | Haemophilus influenzae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.405 | 0.017 | - |
NADPH | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.038 | - |
(2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.039 | - |
NADPH | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.044 | - |
NADPH | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.076 | - |
D-ribulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.19 | - |
D-ribulose 5-phosphate | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.27 | - |
D-ribulose 5-phosphate | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.6 | - |
NADH | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 1.1 | - |
D-ribulose | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 2.2 | - |
D-ribose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 3 | - |
D-arabinose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 3.5 | - |
D-xylulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
2.7.7.40 | 0.092 | - |
CTP | pH 7.2, 25°C, K386A mutant | Haemophilus influenzae | |
2.7.7.40 | 0.099 | - |
CTP | pH 7.2, 25°C | Haemophilus influenzae | |
2.7.7.40 | 0.12 | - |
D-ribitol 5-phosphate | pH 7.2, 25°C, K386A mutant | Haemophilus influenzae | |
2.7.7.40 | 0.13 | - |
D-ribitol 5-phosphate | pH 7.2, 25°C | Haemophilus influenzae | |
2.7.7.40 | 0.39 | - |
CTP | pH 7.2, 25°C, R18A mutant | Haemophilus influenzae | |
2.7.7.40 | 1 | - |
D-ribitol 5-phosphate | pH 7.2, 25°C, R18A mutant | Haemophilus influenzae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.40 | CTP + D-ribitol 5-phosphate | Haemophilus influenzae | - |
diphosphate + CDPribitol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.405 | Haemophilus influenzae | Q48230 | additionally catalyzes the reaction of ribitol-5-phosphate cytidylyltransferase, EC 2.7.7.40 | - |
2.7.7.40 | Haemophilus influenzae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.40 | - |
Haemophilus influenzae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.405 | D-arabinose 5-phosphate + NADPH + H+ | - |
Haemophilus influenzae | ? + NADP+ | - |
? | |
1.1.1.405 | D-erythrose 4-phosphate + NADPH + H+ | - |
Haemophilus influenzae | ? + NADP+ | - |
? | |
1.1.1.405 | D-ribose 5-phosphate + NADPH + H+ | - |
Haemophilus influenzae | ? + NADP+ | - |
? | |
1.1.1.405 | D-ribulose + NADPH + H+ | - |
Haemophilus influenzae | D-ribitol + NADP+ | - |
? | |
1.1.1.405 | D-ribulose 5-phosphate + NADPH + H+ | - |
Haemophilus influenzae | D-ribitol 5-phosphate + NADP+ | - |
? | |
1.1.1.405 | D-xylulose 5-phosphate + NADH + H+ | - |
Haemophilus influenzae | ? + NAD+ | - |
? | |
1.1.1.405 | additional information | the synthase first catalyzes the reduction of D-ribulose 5-phosphate followed by cytidylyl transfer to D-ribitol 5-phosphate, with a 650fold kinetic preference for cytidylyl transfer to D-ribitol 5-phosphate over D-ribulose 5-phosphate | Haemophilus influenzae | ? | - |
? | |
2.7.7.40 | CTP + D-ribitol 5-phosphate | - |
Haemophilus influenzae | diphosphate + CDPribitol | - |
? | |
2.7.7.40 | additional information | enzyme has also ribitol 5-phosphate dehydrogenase activity | Haemophilus influenzae | ? | - |
? | |
2.7.7.40 | additional information | no activity with ATP, GTP, UTP, ADP or CDP | Haemophilus influenzae | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.405 | Bcs1 | - |
Haemophilus influenzae |
1.1.1.405 | bifunctional ribulose 5-phosphate reductase/CDP-ribitol pyrophosphorylase | - |
Haemophilus influenzae |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.405 | 0.32 | - |
D-ribulose 5-phosphate | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.5 | - |
(2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 2.3 | - |
D-arabinose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 3.6 | - |
D-xylulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 3.6 | - |
D-ribulose | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 4.1 | - |
D-ribose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 6.8 | - |
D-ribulose 5-phosphate | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 22 | - |
NADPH | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 22 | - |
D-ribulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
2.7.7.40 | 0.14 | - |
D-ribitol 5-phosphate | pH 7.2, 25°C, R18A mutant | Haemophilus influenzae | |
2.7.7.40 | 9.7 | - |
D-ribitol 5-phosphate | pH 7.2, 25°C, K386A mutant | Haemophilus influenzae | |
2.7.7.40 | 13 | - |
D-ribitol 5-phosphate | pH 7.2, 25°C, wild-type enzyme | Haemophilus influenzae |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.405 | 0.00008 | - |
D-arabinose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.0001 | - |
D-xylulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.0002 | - |
D-ribose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.0002 | - |
D-ribulose 5-phosphate | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.0003 | - |
D-ribulose | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.0008 | - |
NADPH | mutant K386A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.0013 | - |
(2R,3R)-2,3-dihydroxy-4-oxobutyl-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.0025 | - |
D-ribulose 5-phosphate | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.029 | - |
D-ribulose 5-phosphate | wild-type, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.04 | - |
NADPH | mutant R18A, pH 7.2, 25°C | Haemophilus influenzae | |
1.1.1.405 | 0.05 | - |
NADPH | wild-type, pH 7.2, 25°C | Haemophilus influenzae |