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Literature summary extracted from

  • Ghalambor, M.A.; Heath, E.H.
    The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. IV. Purification and properties of cytidine monophosphate 3-deoxy-D-manno-octulosonate synthetase (1966), J. Biol. Chem., 241, 3216-3221.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.38 GSH
-
Escherichia coli

General Stability

EC Number General Stability Organism
2.7.7.38 repeated freezing and thawing results in substantial loss in activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.38 0.22
-
CTP pH 8.0, 37°C Escherichia coli
2.7.7.38 0.8
-
3-deoxy-D-manno-octulosonate pH 8.0, 37°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.38 Mg2+ absolute requirement for a divalent cation Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Escherichia coli biosynthesis of cell wall lipopolysaccharide diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate Escherichia coli 0111-B4 biosynthesis of cell wall lipopolysaccharide diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.38 Escherichia coli
-
-
-
2.7.7.38 Escherichia coli 0111-B4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.38
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.38 50
-
-
Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.7.38 -1°C, enzyme preparation is most satisfactorily stored since inactivation of the enzyme under this condition is negligible over a period of several months Escherichia coli
2.7.7.38 -20°C,partially purified enzyme relatively stable, approximately 20% of the activity is lost after storage for 1 year Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate biosynthesis of cell wall lipopolysaccharide Escherichia coli diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate
-
Escherichia coli 0111-B4 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r
2.7.7.38 CTP + 3-deoxy-D-manno-octulosonate biosynthesis of cell wall lipopolysaccharide Escherichia coli 0111-B4 diphosphate + CMP-3-deoxy-D-manno-octulosonate
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.38 7.8
-
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.38 6.5 9 about half-maximal activity at pH 7.0 and pH 8.4 Escherichia coli