EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.38 | GSH | - |
Escherichia coli |
EC Number | General Stability | Organism |
---|---|---|
2.7.7.38 | repeated freezing and thawing results in substantial loss in activity | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.7.38 | 0.22 | - |
CTP | pH 8.0, 37°C | Escherichia coli | |
2.7.7.38 | 0.8 | - |
3-deoxy-D-manno-octulosonate | pH 8.0, 37°C | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.7.38 | Mg2+ | absolute requirement for a divalent cation | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.38 | CTP + 3-deoxy-D-manno-octulosonate | Escherichia coli | biosynthesis of cell wall lipopolysaccharide | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
2.7.7.38 | CTP + 3-deoxy-D-manno-octulosonate | Escherichia coli 0111-B4 | biosynthesis of cell wall lipopolysaccharide | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.38 | Escherichia coli | - |
- |
- |
2.7.7.38 | Escherichia coli 0111-B4 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.38 | - |
Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.7.38 | 50 | - |
- |
Escherichia coli |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.7.38 | -1°C, enzyme preparation is most satisfactorily stored since inactivation of the enzyme under this condition is negligible over a period of several months | Escherichia coli |
2.7.7.38 | -20°C,partially purified enzyme relatively stable, approximately 20% of the activity is lost after storage for 1 year | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.38 | CTP + 3-deoxy-D-manno-octulosonate | - |
Escherichia coli | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
2.7.7.38 | CTP + 3-deoxy-D-manno-octulosonate | biosynthesis of cell wall lipopolysaccharide | Escherichia coli | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
2.7.7.38 | CTP + 3-deoxy-D-manno-octulosonate | - |
Escherichia coli 0111-B4 | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r | |
2.7.7.38 | CTP + 3-deoxy-D-manno-octulosonate | biosynthesis of cell wall lipopolysaccharide | Escherichia coli 0111-B4 | diphosphate + CMP-3-deoxy-D-manno-octulosonate | - |
r |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.38 | 7.8 | - |
- |
Escherichia coli |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.7.38 | 6.5 | 9 | about half-maximal activity at pH 7.0 and pH 8.4 | Escherichia coli |