Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pastuszak, I.; Ketchum, C.; Hermanson, G.; Sjoberg, E.J.; Drake, R.; Elbein, A.D.
    GDP-L-fucose pyrophosphorylase. Purification, cDNA cloning, and properties of the enzyme (1998), J. Biol. Chem., 273, 30165-30174.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.30 expression of cDNA in murine myeloma cell line, i.e. NSO cells, and in Sf9 cells Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.30 0.054
-
 GTP pH 7.8, 37°C Sus scrofa
2.7.7.30 0.06
-
 fucose 1-phosphate pH 7.8, 37°C Sus scrofa
2.7.7.30 0.12
-
 GDP-L-fucose pH 7.8, 37°C Sus scrofa
2.7.7.30 0.135
-
 diphosphate pH 7.8, 37°C Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.30 Co2+ similar activation as with Mn2+ at 6 mM Sus scrofa
2.7.7.30 Mg2+ divalent cation required for activity, maximum activity at 6-8 mM Mg2+ Sus scrofa
2.7.7.30 Mn2+ approx. 70% of activity with Mg2+ at 4 mM, inhibition above Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.30 61000
-
 gel filtration Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.30 Cricetulus griseus
-
-
-
2.7.7.30 Homo sapiens O14772
-
-
2.7.7.30 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.30 polyethylene glycol, DE-52, Phenyl-Sepharose, hydroxylapatite, Sephacryl S-300, Red-Sepharose, chromatofocusing Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.30 aorta
-
 Sus scrofa
-
2.7.7.30 CHO cell
-
 Cricetulus griseus
-
2.7.7.30 colon mRNA detected by northern analysis Homo sapiens
-
2.7.7.30 heart
-
 Sus scrofa
-
2.7.7.30 kidney
-
 Sus scrofa
-
2.7.7.30 liver
-
 Sus scrofa
-
2.7.7.30 lung
-
 Sus scrofa
-
2.7.7.30 ovary mRNA detected by northern analysis Homo sapiens
-
2.7.7.30 pancreas
-
 Sus scrofa
-
2.7.7.30 prostate mRNA detected by northern analysis Homo sapiens
-
2.7.7.30 small intestine mRNA detected by northern analysis Homo sapiens
-
2.7.7.30 spleen
-
 Sus scrofa
-
2.7.7.30 spleen mRNA detected by northern analysis Homo sapiens
-
2.7.7.30 testis mRNA detected by northern analysis Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
2.7.7.30 -20°C, 50 mM sucrose, several months, no loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.30 ATP + L-fucose 1-phosphate approx. 50% of activity with Sus scrofa diphosphate + ADP-L-fucose
-
 ?
2.7.7.30 GTP + D-arabinose 1-phosphate 5-10% of activity with L-fucose Sus scrofa GDP-D-arabinopyranoside + diphosphate
-
 ?
2.7.7.30 GTP + L-fucose 1-phosphate
-
 Sus scrofa diphosphate + GDP-L-fucose
-
 r
2.7.7.30 ITP + L-fucose 1-phosphate
-
 Sus scrofa diphosphate + IDP-L-fucose
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.30 monomer 1 * 61000, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.30 6.8 7.8
-
 Sus scrofa