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Literature summary extracted from

  • Verachtert, H.; Rodriguez, P.; Bass, S.T.; Hansen, R.G.
    Purification and properties of guanosine diphosphate hexose pyrophosphorylase from mammalian tissues (1966), J. Biol. Chem., 241, 2007-2013.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.28 D-mannose 1-phosphate strong Bos taurus
2.7.7.28 GDPmannose strong Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.28 0.0001
-
 GDPmannose pH 7.8, 25°C Bos taurus
2.7.7.28 0.082
-
 diphosphate pH 7.8, 25°C Bos taurus
2.7.7.28 0.62
-
 GDPglucose pH 7.8, 25°C Bos taurus
2.7.7.28 2.6
-
 GTP pH 7.8, 25°C Bos taurus
2.7.7.28 2.6
-
 D-glucose 1-phosphate pH 7.8, 25°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.7.28 cytoplasm
-
 Rattus norvegicus 5737
-
2.7.7.28 cytoplasm
-
 Bos taurus 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.28 Co2+ metal requirement can be met by Co2+ Bos taurus
2.7.7.28 Mg2+ requirement, maximal activity at about 5 mM, maximal activity at about twice the diphosphate-concentration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.28 GDP-glucose + diphosphate Bos taurus best substrate GTP + D-glucose 1-phosphate
-
 r
2.7.7.28 GDPmannose + diphosphate Bos taurus
-
 GTP + D-mannose 1-phosphate
-
 r
2.7.7.28 GDPmannose + diphosphate Rattus norvegicus reaction at about half the rate of GDPglucose GTP + D-mannose 1-phosphate
-
 ?
2.7.7.28 IDP-glucose + diphosphate Bos taurus reaction at 21% the rate of GDPglucose ITP + D-glucose 1-phosphate
-
 r
2.7.7.28 IDPmannose + diphosphate Bos taurus
-
 ITP + D-mannose 1-phosphate
-
 r
2.7.7.28 IDPmannose + diphosphate Rattus norvegicus reaction at 7% the rate of GDPglucose ITP + D-mannose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.28 Bos taurus
-
-
-
2.7.7.28 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.28
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.7.28 brain
-
 Rattus norvegicus
-
2.7.7.28 kidney
-
 Rattus norvegicus
-
2.7.7.28 liver
-
 Rattus norvegicus
-
2.7.7.28 liver
-
 Bos taurus
-
2.7.7.28 mammary gland
-
 Rattus norvegicus
-
2.7.7.28 muscle
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.7.28 0.38
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.7.28 4°C, at pH 6-6.5, t1/2: 60 days Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.28 GDP-glucose + diphosphate best substrate Bos taurus GTP + D-glucose 1-phosphate
-
 r
2.7.7.28 GDPmannose + diphosphate
-
 Bos taurus GTP + D-mannose 1-phosphate
-
 r
2.7.7.28 GDPmannose + diphosphate reaction at about half the rate of GDPglucose Rattus norvegicus GTP + D-mannose 1-phosphate
-
 ?
2.7.7.28 IDP-glucose + diphosphate reaction at 21% the rate of GDPglucose Bos taurus ITP + D-glucose 1-phosphate
-
 r
2.7.7.28 IDPmannose + diphosphate
-
 Bos taurus ITP + D-mannose 1-phosphate
-
 r
2.7.7.28 IDPmannose + diphosphate reaction at 7% the rate of GDPglucose Rattus norvegicus ITP + D-mannose 1-phosphate
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.7.28 50
-
 15% loss of activity after 5 min, 33% loss of activity after 10 min Bos taurus
2.7.7.28 55
-
 1 min, 57% loss of activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.28 7.5 8
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.7.28 6.5 9 pH 6.5: about 40% of maximal activity, pH 9.0: about half-maximal activity Rattus norvegicus
2.7.7.28 6.5 9 pH 6.5: about 40% of maximal activity, pH 9.0: about half-maximal activity Bos taurus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.7.28 6 6.5 t1/2: 60 days at 4°C Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.7.28 0.00017
-
 GDP-mannose pH 7.8, 25°C Bos taurus
2.7.7.28 0.047
-
 D-mannose 1-phosphate pH 7.8, 25°C Bos taurus