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Literature summary extracted from

  • Nishino, T.; Murao, S.
    Physicochemical and enzymatic properties of ATP:nucleotide pyrophosphotransferase (1975), Agric. Biol. Chem., 39, 1827-1833.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.6.4 EDTA 5 mM, 98.5% inhibition Streptomyces adephospholyticus
2.7.6.4 KMnO4 1 mM, 76% inhibition Streptomyces adephospholyticus
2.7.6.4 Na-diphosphate 1 mM, 14% inhibition Streptomyces adephospholyticus
2.7.6.4 SDS 0.1%, 87% inhibition Streptomyces adephospholyticus
2.7.6.4 tetracyclin 0.5 mg/ml, 37% inhibition Streptomyces adephospholyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.6.4 extracellular
-
 Streptomyces adephospholyticus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.4 Co2+ absolute requirement for a divalent metal ion, at pH 10.0 Mg2+ and Mn2+ are most effective, at pH 7.0 Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus
2.7.6.4 Fe2+ absolute requirement for a divalent metal ion, at pH 10.0 Mg2+ and Mn2+ are most effective, at pH 7.0 Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus
2.7.6.4 Mg2+ absolute requirement for a divalent metal ion, at pH 10.0, Mg2+ and Mn2+ are most effective. At pH 7.0, Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus
2.7.6.4 Mn2+ absolute requirement for a divalent metal ion, at pH 10.0 Mg2+ and Mn2+ are most effective, at pH 7.0 Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus
2.7.6.4 Zn2+ absolute requirement for a divalent metal ion, at pH 10.0 Mg2+ and Mn2+ are most effective, at pH 7.0 Mg2+, Co2+, Mn2+, Fe2+ and Zn2+ are effective Streptomyces adephospholyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.6.4 28000
-
 1 * 28000, SDS-PAGE Streptomyces adephospholyticus
2.7.6.4 29000
-
 equilibrium sedimentation Streptomyces adephospholyticus

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.4 Streptomyces adephospholyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.4 ATP
-
 Streptomyces adephospholyticus AMP + adenosine-3'-diphosphate-5'-triphosphate
-
 ?
2.7.6.4 ATP + ADP
-
 Streptomyces adephospholyticus AMP + adenosine 3'-diphosphate 5'-diphosphate
-
 ?
2.7.6.4 ATP + GDP
-
 Streptomyces adephospholyticus AMP + guanosine 3'-diphosphate 5'-diphosphate
-
 ?
2.7.6.4 ATP + GMP
-
 Streptomyces adephospholyticus AMP + guanosine 3'-phosphate 5'-diphosphate
-
 ?
2.7.6.4 ATP + GTP
-
 Streptomyces adephospholyticus AMP + guanosine-3'-diphosphate-5'-triphosphate
-
 ?
2.7.6.4 ATP + IDP
-
 Streptomyces adephospholyticus AMP + inosine 3'-diphosphate 5'-diphosphate
-
 ?
2.7.6.4 ATP + IMP
-
 Streptomyces adephospholyticus AMP + inosine 3'-diphosphate 5'-phosphate
-
 ?
2.7.6.4 ATP + ITP
-
 Streptomyces adephospholyticus AMP + inosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.4 dATP + ADP
-
 Streptomyces adephospholyticus dAMP + adenosine 3'-diphosphate 5'-diphosphate
-
 ?
2.7.6.4 dATP + ATP
-
 Streptomyces adephospholyticus dAMP + adenosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.4 dATP + GDP
-
 Streptomyces adephospholyticus dAMP + guanosine 3'-diphosphate 5'-diphosphate
-
 ?
2.7.6.4 dATP + GMP
-
 Streptomyces adephospholyticus dAMP + guanosine 3'-diphosphate 5'-phosphate
-
 ?
2.7.6.4 dATP + GTP
-
 Streptomyces adephospholyticus dAMP + guanosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.4 dATP + IDP
-
 Streptomyces adephospholyticus dAMP + inosine 3'-diphosphate 5'-triphosphate
-
 ?
2.7.6.4 dATP + IMP
-
 Streptomyces adephospholyticus dAMP + inosine 3'-diphosphate 5'-phosphate
-
 ?
2.7.6.4 dATP + ITP
-
 Streptomyces adephospholyticus dAMP + inosine 3'-diphosphate 5'-triphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.6.4 monomer 1 * 28000, SDS-PAGE Streptomyces adephospholyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.6.4 6 8 optimal pH with Co2+ as stimulating cation Streptomyces adephospholyticus
2.7.6.4 10 10.5 optimal pH with Mg2+ or Mn2+ as stimulating cation Streptomyces adephospholyticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.6.4 Streptomyces adephospholyticus isoelectric focusing
-
 7.7