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Literature summary extracted from

  • Petit, C.M.; Koretke, K.K.
    Characterization of Streptococcus pneumoniae thymidylate kinase: steady-state kinetics of the forward reaction and isothermal titration calorimetry (2002), Biochem. J., 363, 825-831.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.9 histidine-tagged enzyme, overexpression in Escherichia coli Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 ADP ADP[beta-S] Streptococcus pneumoniae
2.7.4.9 dTDP
-
 Streptococcus pneumoniae
2.7.4.9 dTMP
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 0.066
-
 dTMP pH 8.5, 25°C Streptococcus pneumoniae
2.7.4.9 0.235
-
 ATP pH 8.5, 25°C Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.9 ATP + dTMP Streptococcus pneumoniae reaction in both the de novo and salvage pathways of dTTP synthesis ADP + dTDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Streptococcus pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.9
-
 Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + 3'-azido-3'-deoxythymidine 5'-monophosphate no activity Streptococcus pneumoniae ADP + 3'-azido-3'-deoxythymidine 5'-diphosphate
-
 ?
2.7.4.9 ATP + dTMP
-
 Streptococcus pneumoniae ADP + dTDP
-
 ?
2.7.4.9 ATP + dTMP reaction in both the de novo and salvage pathways of dTTP synthesis Streptococcus pneumoniae ADP + dTDP
-
 ?
2.7.4.9 ATP + dUMP 15% of the activity with dTMP Streptococcus pneumoniae ADP + dUDP
-
 ?
2.7.4.9 CTP + dTMP 20% of the activity with ATP Streptococcus pneumoniae CDP + dTDP
-
 ?
2.7.4.9 dATP + dTMP 80% of the activity with ATP Streptococcus pneumoniae dADP + dTDP
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.9 8.9
-
 ATP pH 8.5, 25°C Streptococcus pneumoniae
2.7.4.9 8.9
-
 dTMP pH 8.5, 25°C Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.9 8.5
-
-
 Streptococcus pneumoniae

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.9 7 9.5 pH 7.0: about 40% of maximal activity, pH 9.5: about 55% of maximal activity Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.9 0.047
-
 ADP pH 8.5, 25°C, with ATP as variable substrate Streptococcus pneumoniae
2.7.4.9 0.16
-
 dTMP pH 8.5, 25°C, dTMP as variable substrate Streptococcus pneumoniae
2.7.4.9 0.415
-
 ADP pH 8.5, 25°C, with dTMP as variable substrate Streptococcus pneumoniae