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Literature summary extracted from

  • Nelson, D.J.; Carter, C.E.
    Purification and characterization of Thymidine 5-monophosphate kinase from Escherichia coli B (1969), J. Biol. Chem., 244, 5254-5262.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 5-iodo-2'-dUMP
-
 Escherichia coli
2.7.4.9 dCTP
-
 Escherichia coli
2.7.4.9 dTTP
-
 Escherichia coli
2.7.4.9 dUMP
-
 Escherichia coli
2.7.4.9 NaCl 0.1 M, 25% inhibition Escherichia coli
2.7.4.9 NH4Cl 0.4 M, 75% inhibition Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 0.24
-
 dTMP pH 7.8, 37°C Escherichia coli
2.7.4.9 1.2
-
 ATP pH 7.8, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.9 Co2+ 18% of maximal activation with Mg2+ Escherichia coli
2.7.4.9 Mg2+ absolute requirement for divalent cation. When Mg2+ is equal to ATP, the rate of dTMP kinase reaction is maximal Escherichia coli
2.7.4.9 Mn2+ 41% of maximal activation with Mg2+ Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.9 65000
-
 gel filtration, sucrose density gradient centrifugation Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Escherichia coli
-
-
-
2.7.4.9 Escherichia coli B / ATCC 11303
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.9
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.4.9 -70°C, 1 year, 20-30% loss of activity Escherichia coli
2.7.4.9 2°C, half-life of about 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + 5-iodo-2'-dUMP 60% of the maximal activity with dTMP Escherichia coli ADP + 5-iodo-2'-dUDP
-
 ?
2.7.4.9 ATP + dTMP
-
 Escherichia coli ADP + dTDP
-
 ?
2.7.4.9 ATP + dTMP
-
 Escherichia coli B / ATCC 11303 ADP + dTDP
-
 ?
2.7.4.9 ATP + dUMP at 15% of the activity with dTMP Escherichia coli ADP + dUDP
-
 ?
2.7.4.9 ATP + dUMP at 15% of the activity with dTMP Escherichia coli B / ATCC 11303 ADP + dUDP
-
 ?
2.7.4.9 CTP + dTMP about 30% of the activity with ATP Escherichia coli CDP + dTDP
-
 ?
2.7.4.9 CTP + dTMP about 30% of the activity with ATP Escherichia coli B / ATCC 11303 CDP + dTDP
-
 ?
2.7.4.9 dATP + dTMP about 80% of the activity with ATP Escherichia coli dADP + dTDP
-
 ?
2.7.4.9 dATP + dTMP about 80% of the activity with ATP Escherichia coli B / ATCC 11303 dADP + dTDP
-
 ?
2.7.4.9 dCTP + dTMP about 20% of the activity with ATP Escherichia coli dCDP + dTDP
-
 ?
2.7.4.9 dGTP + dTMP about 10% of maximal activity with ATP Escherichia coli dGDP + dTDP
-
 ?
2.7.4.9 dGTP + dTMP about 10% of maximal activity with ATP Escherichia coli B / ATCC 11303 dGDP + dTDP
-
 ?
2.7.4.9 dTTP + dTMP about 3% of the activity with ATP Escherichia coli dTDP
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.9 7.8
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.9 7 8.7 pH 7.0: about 50% of maximal activity, pH 8.7: about 40% of maximal activity Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.9 0.4
-
 5-iodo-2'-dUMP pH 7.8, 37°C Escherichia coli