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Literature summary extracted from

  • Smith, L.K.; Eakin, R.E.
    Partial purification and characterization of thymidylate kinase from embryonic chick liver (1975), Arch. Biochem. Biophys., 167, 61-71.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.9 the enzyme is very labile and can be stabilized for long periods of time by its substrate thymidine 5'-monophosphate in the presence of 2-mercaptoethanol Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 ADP 25% inhibition Gallus gallus
2.7.4.9 dATP 0.4 mM, 12% inhibition, adult liver Gallus gallus
2.7.4.9 dTDP 0.4 mM, 48% inhibition Gallus gallus
2.7.4.9 dTTP 0.4 mM, 43% inhibition Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 additional information
-
additional information
-
Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.9 Mg2+ requirement of a Mg2+:ATP ratio greater than 1.0 and for optimal activity there is a requirement of an additional 2-3 mM Mg2+ above the concentration of ATP Gallus gallus
2.7.4.9 Mn2+ can partially replace Mg2+ requirement Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.9 46000
-
gel filtration Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.9 partial Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.9 liver embryonic and adult Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.9 0.188
-
-
Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.9 7.5
-
-
Gallus gallus