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Literature summary extracted from

  • Kielley, R.K.
    Purification and properties of thymidine monophosphate kinase from mouse hepatoma (1970), J. Biol. Chem., 245, 4204-4212.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.4.9 dTDP and dTTP are effective as enzyme stabilizer Mus musculus
2.7.4.9 the stability of the enzyme is maintained during purification by the constant presence of dTMP and 2-mercaptoethanol and by the elimination of substrate-destoying phosphatase activity Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 2-mercaptoethanol 0.4 mM Mus musculus
2.7.4.9 ADP 100 nM, 64% inhibition Mus musculus
2.7.4.9 Ca2+
-
Mus musculus
2.7.4.9 CH2ATP 100 nM, 23% inhibition Mus musculus
2.7.4.9 dADP 100 nM, 57% inhibition Mus musculus
2.7.4.9 dATP 100 nM, 15% inhibition Mus musculus
2.7.4.9 dGTP 100 nM, 12% inhibition Mus musculus
2.7.4.9 dTDP 100 nM, 39% inhibition Mus musculus
2.7.4.9 GDP 100 nM, 21% inhibition Mus musculus
2.7.4.9 Mn2+ slight inhibition Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 0.19
-
dTMP pH 7.4, 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.9 Mg2+ required, concentration range for maximal activity is From Mg:ATP ratios of 1 to 3 with an optimum around 1.3 to 1.5 Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.9 the stability of the enzyme is maintained during purification by the constant presence of dTMP and 2-mercaptoethanol and by the elimination of substrate-destoying phosphatase activity Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.9 hepatoma ascites cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.9 12.76
-
-
Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + dTMP
-
Mus musculus ADP + dTDP
-
r
2.7.4.9 ATP + dUMP 23% of the activity with dTMP Mus musculus ADP + dUDP
-
r
2.7.4.9 dATP + dTMP 70.1% of the activity with ATP Mus musculus dADP + dTDP
-
r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.9 7
-
-
Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.9 0.25
-
ADP pH 7.4, 37°C Mus musculus