EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.2 | 0.075 | - |
phosphomevalonate | - |
Sus scrofa | |
2.7.4.2 | 0.46 | - |
ATP | - |
Sus scrofa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.2 | Co2+ | can partially replace Mg2+ in activation | Sus scrofa | |
2.7.4.2 | Co2+ | 63.6% of the activity with Mg2+ | Sus scrofa | |
2.7.4.2 | Mg2+ | maximal activity at 5 mM | Sus scrofa | |
2.7.4.2 | Mg2+ | divalent cation required, Mg2+ most efficient | Sus scrofa | |
2.7.4.2 | Mn2+ | can partially replace Mg2+ in activation | Sus scrofa | |
2.7.4.2 | Mn2+ | 62.2% of the activity with Mg2+ | Sus scrofa | |
2.7.4.2 | Zn2+ | can partially replace Mg2+ in activation | Sus scrofa | |
2.7.4.2 | Zn2+ | 65.1% of the activity with Mg2+ | Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.2 | 21000 | - |
1 * 21000, SDS-PAGE | Sus scrofa |
2.7.4.2 | 22000 | - |
sucrose density gradient centrifugation | Sus scrofa |
2.7.4.2 | 22500 | - |
gel filtration | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.2 | ATP + (R)-5-phosphomevalonate | Sus scrofa | one of the enzymes in the biosynthetic pathway of the polyisoprenoid precursor isopentenyl diphosphate, enzyme has no regulatory function in cholesterol biosynthesis in liver | ADP + 5-diphosphomevalonate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.2 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.4.2 | ammonium sulfate, DEAE-cellulose, BioGel P-150, hydroxylapatite, Blue Dextran-Sepharose | Sus scrofa |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.4.2 | ATP + (R)-5-phosphomevalonate = ADP + (R)-5-diphosphomevalonate | sequential mechanism | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.4.2 | liver | - |
Sus scrofa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.4.2 | 69.1 | - |
- |
Sus scrofa |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.4.2 | -20°C, 10 mM Tris or phosphate buffer, pH 7.5, 50% glycerol, 10 mM 2-mercaptoethanol, 60 days, no loss of activity | Sus scrofa |
2.7.4.2 | -20°C, 50% glycerol | Sus scrofa |
2.7.4.2 | 4°C, 10 mM 2-mercaptoethanol, 3 months, no loss of activity | Sus scrofa |
2.7.4.2 | 4°C, 20 days, 99% loss of activity | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.2 | ATP + (R)-5-phosphomevalonate | one of the enzymes in the biosynthetic pathway of the polyisoprenoid precursor isopentenyl diphosphate, enzyme has no regulatory function in cholesterol biosynthesis in liver | Sus scrofa | ADP + 5-diphosphomevalonate | - |
r | |
2.7.4.2 | ATP + 5-phosphomevalonate | absolute specificity for ATP | Sus scrofa | ADP + 5-diphosphomevalonate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.2 | monomer | 1 * 21000, SDS-PAGE | Sus scrofa |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.2 | 30 | - |
- |
Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.2 | 7.5 | 9.5 | - |
Sus scrofa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.2 | 6 | 9.5 | approx. 30% of maximal activity at pH 6.0 | Sus scrofa |