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Literature summary extracted from

  • Cooper, J.R.; Nishino, K.
    Enzymatic synthesis of thiamin triphosphate (1986), Methods Enzymol., 122, 24-29.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.15 NEM 10 mM, 64% inhibition Bos taurus
2.7.4.15 PCMB 0.5 mM, 79.5% inhibition Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.15 Mg2+ required Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.15 103000
-
-
 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.15 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.15
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.15 brain cortex Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.15 ATP + thiamine diphosphate
-
 Bos taurus ADP + thiamine triphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.15 7.5
-
 in Tris-HCl buffer slightly higher activity than in phosphate buffer Bos taurus