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Literature summary extracted from

  • Liou, J.Y.; Dutschman, G.E.; Lam, W.; Jiang, Z.; Cheng, Y.C.
    Characterization of human UMP/CMP kinase and its phosphorylation of D- and L-form deoxycytidine analogue monophosphates (2002), Cancer Res., 62, 1624-1631.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.14 expression in Escherichia coli. The 228-aa and the 196-11 form are expressed as His-tagged fusion protein. The 196-aa UMP/CMP kinase is the actual form of the enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.14 CTP inhibits reaction with ATP and UMP, CMP or dCMP Homo sapiens
2.7.4.14 dCTP inhibits reaction with ATP and UMP, CMP or dCMP Homo sapiens
2.7.4.14 TTP inhibits reaction with ATP and UMP, CMP or dCMP Homo sapiens
2.7.4.14 UTP inhibits reaction with ATP and UMP, CMP or dCMP Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.14 0.015
-
 CMP 37°C, 196-aa and 228-aa enzyme form, in presence of 8 mM MgATP2- Homo sapiens
2.7.4.14 0.023
-
 dCMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- Homo sapiens
2.7.4.14 0.067
-
 UMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- Homo sapiens
2.7.4.14 0.069
-
 UMP 37°C, 228-aa enzyme form, in presence of 8 mM MgATP2- Homo sapiens
2.7.4.14 0.204
-
 ATP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- Homo sapiens
2.7.4.14 0.211
-
 CMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- Homo sapiens
2.7.4.14 0.228
-
 beta-L-(-)-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP 37°C Homo sapiens
2.7.4.14 0.25
-
 L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP 37°C, His-tagged UMP/CMP kinase Homo sapiens
2.7.4.14 0.272
-
 beta-D-2',3'-dideoxy-CMP 37°C Homo sapiens
2.7.4.14 0.354
-
 dCMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- Homo sapiens
2.7.4.14 0.494
-
 L-(-)-2',3'-dideoxy-3'-thia-CMP 37°C Homo sapiens
2.7.4.14 0.513
-
 dCMP 37°C, 196-aa enzyme form, in presence of 8 mM MgATP2- Homo sapiens
2.7.4.14 0.581
-
 gemcitabine monophosphate 37°C Homo sapiens
2.7.4.14 0.697
-
 beta-L-2',3'-dideoxy-CMP 37°C Homo sapiens
2.7.4.14 0.917
-
 ara-CMP 37°C Homo sapiens
2.7.4.14 1.037
-
 beta-D-2',3'-dideoxy-CMP 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.14 cytoplasm
-
 Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.14 ATP + CMP Homo sapiens the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis ADP + CDP
-
 ?
2.7.4.14 ATP + dCMP Homo sapiens the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis ADP + dCDP
-
 ?
2.7.4.14 ATP + UMP Homo sapiens the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis ADP + UDP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.14 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.14
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.14 HeLa-S3 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.14 ATP + ara-CMP
-
 Homo sapiens ADP + ara-CDP
-
 ?
2.7.4.14 ATP + beta-D-2',3'-dideoxy-CMP + H2O
-
 Homo sapiens ADP + ?
-
 ?
2.7.4.14 ATP + beta-L-2',3'-dideoxy-2',3'-didehydro-5-fluoro-CMP
-
 Homo sapiens ADP + ?
-
 ?
2.7.4.14 ATP + beta-L-2',3'-dideoxy-CMP + H2O
-
 Homo sapiens ADP + ?
-
 ?
2.7.4.14 ATP + beta-L-dioxolane-cytidine
-
 Homo sapiens ADP + ?
-
 ?
2.7.4.14 ATP + CMP
-
 Homo sapiens ADP + CDP
-
 ?
2.7.4.14 ATP + CMP the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis Homo sapiens ADP + CDP
-
 ?
2.7.4.14 ATP + dCMP
-
 Homo sapiens ADP + dCDP
-
 ?
2.7.4.14 ATP + dCMP the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis Homo sapiens ADP + dCDP
-
 ?
2.7.4.14 ATP + gemcitabine monophosphate
-
 Homo sapiens ADP + ?
-
 ?
2.7.4.14 ATP + L-(-)-2',3'-dideoxy-3'-thia-CMP
-
 Homo sapiens ADP + L-(-)-2',3'-dideoxy-3'-thia-CDP
-
 ?
2.7.4.14 ATP + L-(-)-2',3'-dideoxy-5-fluoro-3'-thia-CMP
-
 Homo sapiens ADP + ?
-
 ?
2.7.4.14 ATP + UMP
-
 Homo sapiens ADP + UDP
-
 ?
2.7.4.14 ATP + UMP the enzyme plays a crucial role in the formation of UDP, CDP and dCDP which are required for cellular nucleic acid synthesis Homo sapiens ADP + UDP
-
 ?
2.7.4.14 CTP + CMP
-
 Homo sapiens CDP + CDP
-
 ?
2.7.4.14 dATP + CMP
-
 Homo sapiens dADP + CDP
-
 ?
2.7.4.14 dATP + UMP
-
 Homo sapiens dADP + ADP
-
 ?
2.7.4.14 dCTP + dCMP
-
 Homo sapiens dCDP + dCDP
-
 ?
2.7.4.14 dCTP + UMP
-
 Homo sapiens dGDP + UDP
-
 ?
2.7.4.14 dGTP + CMP
-
 Homo sapiens dGDP + CDP
-
 ?
2.7.4.14 dGTP + UMP
-
 Homo sapiens dGDP + UDP
-
 ?
2.7.4.14 GTP + CMP
-
 Homo sapiens GDP + CDP
-
 ?
2.7.4.14 GTP + dCMP
-
 Homo sapiens GDP + dCDP
-
 ?
2.7.4.14 GTP + UMP
-
 Homo sapiens GDP + UDP
-
 ?
2.7.4.14 TTP + CMP
-
 Homo sapiens TDP + CDP
-
 ?
2.7.4.14 TTP + dCMP
-
 Homo sapiens TDP + dCMP
-
 ?
2.7.4.14 TTP + UMP
-
 Homo sapiens TDP + UDP
-
 ?