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Literature summary extracted from

  • Kameda, A.; Shiba, T.; Kawazoe, Y.; Satoh, Y.; Ihara, Y.; Munekata, M.; Ishige, K.; Noguchi, T.
    A novel ATP regeneration system using polyphosphate-AMP phosphotransferase and polyphosphate kinase (2001), J. Biosci. Bioeng., 91, 557-563.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.4.1 synthesis used for an ATP regeneration system for acetyl-CoA synthesis Escherichia coli
2.7.4.33 synthesis design of an ATP regeneration system with poylphosphate-AMP phosphotransferase and polyphosphate kinase. The ATP regeneration system can also be used as a GTP regeneration system Myxococcus xanthus
2.7.4.33 synthesis the phospho-conversion of AMP to ADP using polyphosphate as phosphate donor by polyphosphate-AMP phosphotransferase, PAP, followed by the synthesis of ATP from ADP through poylphosphate kinase, PPK, is used as an ATP regenrating system. The PAP-PPK ATP regeneration system can continously produce ATP in the coupled reaction from polyphosphate, whhich is a cheaper substrate than acetylphosphate, phosphoenolphosphate, or creatine phosphate. The system can also regenerate GTP from GMP. Method optimization, overview. ATP can be used for e.g. synthesis of acetly-CoA Myxococcus xanthus
2.7.4.33 synthesis the phospho-conversion of AMP to ADP using polyphosphate as phosphate donor by polyphosphate-AMP phosphotransferase, PAP, followed by the synthesis of ATP from ADP through poylphosphate kinase, PPK, is used as an ATP regenrating system. The PAP-PPK ATP regeneration system can continously produce ATP in the coupled reaction from polyphosphate, whhich is a cheaper substrate than acetylphosphate, phosphoenolphosphate, or creatine phosphate. The system can also regenerate GTP from GMP. Method optimization, overview. ATP can be used for e.g. synthesis of acetly-CoA Acinetobacter johnsonii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.33 membrane
-
 Myxococcus xanthus 16020
-
2.7.4.33 soluble
-
 Acinetobacter johnsonii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.33 Mg2+ required Myxococcus xanthus
2.7.4.33 Mg2+ required Acinetobacter johnsonii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.33 AMP + [phosphate]n Myxococcus xanthus
-
 ADP + [phosphate]n-1
-
 ?
2.7.4.33 AMP + [phosphate]n Acinetobacter johnsonii
-
 ADP + [phosphate]n-1
-
 ?
2.7.4.33 AMP + [phosphate]n Myxococcus xanthus DK101
-
 ADP + [phosphate]n-1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Escherichia coli
-
-
-
2.7.4.33 Acinetobacter johnsonii
-
-
-
2.7.4.33 Myxococcus xanthus
-
-
-
2.7.4.33 Myxococcus xanthus DK101
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.33 native enzyme 200fold by membrane fraction preparation and washing with detergent Triton X-100 and SB-12 Myxococcus xanthus
2.7.4.33 partial Myxococcus xanthus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.33 12
-
 partially purified native enzyme, pH 8.0, 37°C, ADP synthesis Myxococcus xanthus

Storage Stability

EC Number Storage Stability Organism
2.7.4.33 4°C, membrane fraction, over 2 months, stable Myxococcus xanthus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.33 AMP + (phosphate)n
-
 Myxococcus xanthus ADP + (phosphate)n-1
-
 ?
2.7.4.33 AMP + (phosphate)n
-
 Myxococcus xanthus DK101 ADP + (phosphate)n-1
-
 ?
2.7.4.33 AMP + [phosphate]n
-
 Myxococcus xanthus ADP + [phosphate]n-1
-
 ?
2.7.4.33 AMP + [phosphate]n
-
 Acinetobacter johnsonii ADP + [phosphate]n-1
-
 ?
2.7.4.33 AMP + [phosphate]n
-
 Myxococcus xanthus DK101 ADP + [phosphate]n-1
-
 ?
2.7.4.33 GMP + [phosphate]n
-
 Myxococcus xanthus GDP + [phosphate]n-1
-
 ?
2.7.4.33 GMP + [phosphate]n
-
 Acinetobacter johnsonii GDP + [phosphate]n-1
-
 ?
2.7.4.33 GMP + [phosphate]n
-
 Myxococcus xanthus DK101 GDP + [phosphate]n-1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.33 PAP
-
 Myxococcus xanthus
2.7.4.33 PAP
-
 Acinetobacter johnsonii
2.7.4.33 poylphosphate-AMP phosphotransferase
-
 Myxococcus xanthus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.4.33 37
-
 assay at Myxococcus xanthus
2.7.4.33 37
-
 assay at Acinetobacter johnsonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.33 8
-
 assay at Myxococcus xanthus
2.7.4.33 8
-
 assay at Acinetobacter johnsonii