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Literature summary extracted from

  • Tzeng, C.M.; Kornberg, A.
    The multiple activities of polyphosphate kinase of Escherichia coli and their subunit structure determined by radiation target analysis (2000), J. Biol. Chem., 275, 3977-3983.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.1 diphosphate 10 mM, 100% activation of guanosine 5'-tetraphosphate synthesis Escherichia coli
2.7.4.1 Guanidine HCl 5 mM, 20% activation of guanosine 5'-tetraphosphate synthesis Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.4.1 F488A complete loss of all 4 polyphosphate kinase activities Escherichia coli
2.7.4.1 P507A complete loss of all 4 polyphosphate kinase activities Escherichia coli
2.7.4.1 Q674A complete loss of all 4 polyphosphate kinase activities Escherichia coli
2.7.4.1 R375A complete loss of all 4 polyphosphate kinase activities Escherichia coli
2.7.4.1 R564A complete loss of all 4 polyphosphate kinase activities Escherichia coli
2.7.4.1 R621A complete loss of all 4 polyphosphate kinase activities Escherichia coli
2.7.4.1 S380A complete loss of all 4 polyphosphate kinase activities Escherichia coli
2.7.4.1 Y468A 120-140% of wild-type polyphosphate, GTP, and guanosine 5'-tetraphosphate synthesis activity, 20-50% of wild-type ATP synthesis activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.1 diphosphate 10 mM, 66% inhibition of polyphosphate synthesis, 75% inhibition of GTP synthesis Escherichia coli
2.7.4.1 GMP competitive inhibition of polyphosphate 750 and GDP in guanosine 5'-tetraphosphate synthesis Escherichia coli
2.7.4.1 Guanidine HCl 5 mM, 50% inhibition of polyphosphate synthesis Escherichia coli
2.7.4.1 Polyphosphate 65 residues, competitive inhibition of polyphosphate 750 and GDP in guanosine 5'-tetraphosphate synthesis Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.1 0.035
-
Polyphosphate pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis Escherichia coli
2.7.4.1 0.16
-
GDP pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.1 Mg2+ maximal activity of polyphosphate synthesis, ATP and GTP synthesis, and guanosine 5'-tetraphosphate synthesis at 5.0, 2.0, 1.0 and 0.2 mM Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.1 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.1 ATP + (phosphate)n
-
Escherichia coli ADP + (phosphate)n+1 polyphosphate chains of lenths of 700-800 residues ?
2.7.4.1 GDP + (phosphate)n+1
-
Escherichia coli GTP + (phosphate)n
-
?
2.7.4.1 GDP + (phosphate)n+2
-
Escherichia coli guanosine 5'-tetraphosphate + (phosphate)n
-
?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.1 PPK1 i.e. polyphosphate kinase 1 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.1 0.482
-
GDP pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis Escherichia coli
2.7.4.1 1.04
-
Polyphosphate pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.4.1 0.0007
-
GMP pH 7.5, 37°C, competitive inhibition of GDP in guanosine 5'-tetraphosphate synthesis Escherichia coli
2.7.4.1 0.0012
-
polyphosphate 65 pH 7.5, 37°C, competitive inhibition of GDP in guanosine 5'-tetraphosphate synthesis Escherichia coli
2.7.4.1 0.0014
-
GMP pH 7.5, 37°C, competitive inhibition of polyphosphate 750 in guanosine 5'-tetraphosphate synthesis Escherichia coli
2.7.4.1 0.0076
-
polyphosphate 65 pH 7.5, 37°C, competitive inhibition of polyphosphate 750 in guanosine 5'-tetraphosphate synthesis Escherichia coli