EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.1 | diphosphate | 10 mM, 100% activation of guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
2.7.4.1 | Guanidine HCl | 5 mM, 20% activation of guanosine 5'-tetraphosphate synthesis | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.4.1 | F488A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
2.7.4.1 | P507A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
2.7.4.1 | Q674A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
2.7.4.1 | R375A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
2.7.4.1 | R564A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
2.7.4.1 | R621A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
2.7.4.1 | S380A | complete loss of all 4 polyphosphate kinase activities | Escherichia coli |
2.7.4.1 | Y468A | 120-140% of wild-type polyphosphate, GTP, and guanosine 5'-tetraphosphate synthesis activity, 20-50% of wild-type ATP synthesis activity | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.1 | diphosphate | 10 mM, 66% inhibition of polyphosphate synthesis, 75% inhibition of GTP synthesis | Escherichia coli | |
2.7.4.1 | GMP | competitive inhibition of polyphosphate 750 and GDP in guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
2.7.4.1 | Guanidine HCl | 5 mM, 50% inhibition of polyphosphate synthesis | Escherichia coli | |
2.7.4.1 | Polyphosphate | 65 residues, competitive inhibition of polyphosphate 750 and GDP in guanosine 5'-tetraphosphate synthesis | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.1 | 0.035 | - |
Polyphosphate | pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
2.7.4.1 | 0.16 | - |
GDP | pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.1 | Mg2+ | maximal activity of polyphosphate synthesis, ATP and GTP synthesis, and guanosine 5'-tetraphosphate synthesis at 5.0, 2.0, 1.0 and 0.2 mM | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.1 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.1 | ATP + (phosphate)n | - |
Escherichia coli | ADP + (phosphate)n+1 | polyphosphate chains of lenths of 700-800 residues | ? | |
2.7.4.1 | GDP + (phosphate)n+1 | - |
Escherichia coli | GTP + (phosphate)n | - |
? | |
2.7.4.1 | GDP + (phosphate)n+2 | - |
Escherichia coli | guanosine 5'-tetraphosphate + (phosphate)n | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.1 | PPK1 | i.e. polyphosphate kinase 1 | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.1 | 0.482 | - |
GDP | pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
2.7.4.1 | 1.04 | - |
Polyphosphate | pH 7.5, 37°C, guanosine 5'-tetraphosphate synthesis | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.4.1 | 0.0007 | - |
GMP | pH 7.5, 37°C, competitive inhibition of GDP in guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
2.7.4.1 | 0.0012 | - |
polyphosphate 65 | pH 7.5, 37°C, competitive inhibition of GDP in guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
2.7.4.1 | 0.0014 | - |
GMP | pH 7.5, 37°C, competitive inhibition of polyphosphate 750 in guanosine 5'-tetraphosphate synthesis | Escherichia coli | |
2.7.4.1 | 0.0076 | - |
polyphosphate 65 | pH 7.5, 37°C, competitive inhibition of polyphosphate 750 in guanosine 5'-tetraphosphate synthesis | Escherichia coli |